Abstract
Four individual lines and one subline of human cells, permanently established in tissue culture after infection with simian virus 40, failed to form tumors when inoculated into athymic nude mice. Under identical conditions, three established human cell lines of neoplastic origin and a spontaneously established human lymphocyte line formed tumors. Nude mice that failed to grow tumors from inocula of simian virus 40-transformed human cells, grew tumors from subsequent injections of authentic human cancer cells. Further efforts to demonstrate an immunologic basis for the growth suppression of human simian virus 40 transformants were also negative. The data suggest that the changes in morphology and in vitro growth behavior induced by the viral information are not sufficient for, or are only coincidentally related to, the neoplastic state.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Butel J. S., Tevethia S. S., Melnick J. L. Oncogenicity and cell transformation by papovavirus SV40: the role of the viral genome. Adv Cancer Res. 1972;15:1–55. doi: 10.1016/s0065-230x(08)60371-1. [DOI] [PubMed] [Google Scholar]
- Croce C. M., Aden D., Koprowski H. Somatic cell hybrids between mouse peritoneal macrophages and simian-virus-40-transformed human cells: II. Presence of human chromosome 7 carrying simin virus 40 genome in cells of tumors induced by hybrid cells. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1397–1400. doi: 10.1073/pnas.72.4.1397. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Croce C. M., Girardi A. J., Koprowski H. Assignment of the T-antigen gene of simian virus 40 to human chromosome C-7. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3617–3620. doi: 10.1073/pnas.70.12.3617. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Echkart W. Genetics of DNA tumor viruses. Annu Rev Genet. 1974;8:301–317. doi: 10.1146/annurev.ge.08.120174.001505. [DOI] [PubMed] [Google Scholar]
- Fidler I. J. In vitro studies of cellular-mediated immunostimulation of tumor growth. J Natl Cancer Inst. 1973 May;50(5):1307–1312. doi: 10.1093/jnci/50.5.1307. [DOI] [PubMed] [Google Scholar]
- Flanagan S. P. 'Nude', a new hairless gene with pleiotropic effects in the mouse. Genet Res. 1966 Dec;8(3):295–309. doi: 10.1017/s0016672300010168. [DOI] [PubMed] [Google Scholar]
- Folkman J., Merler E., Abernathy C., Williams G. Isolation of a tumor factor responsible for angiogenesis. J Exp Med. 1971 Feb 1;133(2):275–288. doi: 10.1084/jem.133.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freedman V. H., Shin S. I. Cellular tumorigenicity in nude mice: correlation with cell growth in semi-solid medium. Cell. 1974 Dec;3(4):355–359. doi: 10.1016/0092-8674(74)90050-6. [DOI] [PubMed] [Google Scholar]
- Irvin A. D., Brown C. G., Kanhai G. K., Stagg D. A. Comparative growth of bovine lymphosarcoma cells and lymphoid cells infected with Theileria parva in athymic (nude) mice. Nature. 1975 Jun 26;255(5511):713–714. doi: 10.1038/255713a0. [DOI] [PubMed] [Google Scholar]
- Koene R. A., Gerlag P. G., Jansen J. J., Hagemann J. F., Wijdeveld P. G. Rejection of skin grafts in the nude mouse. Nature. 1974 Sep 6;251(5470):69–70. doi: 10.1038/251069a0. [DOI] [PubMed] [Google Scholar]
- LITTLEFIELD J. W. THREE DEGREES OF GUANYLIC ACID--INOSINIC ACID PYROPHOSPHORYLASE DEFICIENCY IN MOUSE FIBROBLASTS. Nature. 1964 Sep 12;203:1142–1144. doi: 10.1038/2031142a0. [DOI] [PubMed] [Google Scholar]
- Lance E. M. Response of mice to xenografts of skin. Transplant Proc. 1970 Dec;2(4):497–505. [PubMed] [Google Scholar]
- Levy J. A., Virolainen M., Defendi V. Human lymphoblastoid lines from lymph node and spleen. Cancer. 1968 Sep;22(3):517–524. doi: 10.1002/1097-0142(196809)22:3<517::aid-cncr2820220305>3.0.co;2-a. [DOI] [PubMed] [Google Scholar]
- MOORE G. E., SANDBERG A. A. STUDIES OF A HUMAN TUMOR CELL LINE WITH A DIPLOID KARYOTYPE. Cancer. 1964 Feb;17:170–175. doi: 10.1002/1097-0142(196402)17:2<170::aid-cncr2820170206>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
- PONTEN J., JENSEN F., KOPROWSKI H. Morphological and virological investigation of human tissue cultures transformed with SV40. J Cell Comp Physiol. 1963 Apr;61:145–163. doi: 10.1002/jcp.1030610206. [DOI] [PubMed] [Google Scholar]
- Prehn R. T. The immune reaction as a stimulator of tumor growth. Science. 1972 Apr 14;176(4031):170–171. doi: 10.1126/science.176.4031.170. [DOI] [PubMed] [Google Scholar]
- Risser R., Pollack R. A nonselective analysis of SV40 transformation of mouse 3T3 cells. Virology. 1974 Jun;59(2):477–489. doi: 10.1016/0042-6822(74)90457-7. [DOI] [PubMed] [Google Scholar]
- SHEIN H. M., ENDERS J. F. Transformation induced by simian virus 40 in human renal cell cultures. I. Morphology and growth characteristics. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1164–1172. doi: 10.1073/pnas.48.7.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stanbridge E. J., Perkins F. T., Hayflick L. Cell tumourigenicity detected by heterotransplantation into mice immunosuppressed with anti-lymphocytic serum. Prog Immunobiol Stand. 1971;5:243–251. [PubMed] [Google Scholar]
- Stanbridge E. J., Perkins F. T. Tumour nodule formation as an in vivo measure of the suppression of cellular immune response by antilymphocytic serum. Nature. 1969 Jan 4;221(5175):80–81. doi: 10.1038/221080a0. [DOI] [PubMed] [Google Scholar]
- Tordaro G. J., Green H. An assay for cellular transformation by SV40. Virology. 1964 May;23(1):117–119. doi: 10.1016/s0042-6822(64)80018-0. [DOI] [PubMed] [Google Scholar]
- Weiss M. C., Ephrussi B., Scaletta L. J. Loss of T-antigen from somatic hybrids between mouse cells and SV40-transformed human cells. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1132–1135. doi: 10.1073/pnas.59.4.1132. [DOI] [PMC free article] [PubMed] [Google Scholar]