Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Dec;72(12):5086–5089. doi: 10.1073/pnas.72.12.5086

Different responses of lymphoid cells from tumor-bearing as compared to tumor-immunized mice when sensitized to tumor specific antigens in vitro.

M A Kall, I Hellström, K E Hellström
PMCID: PMC388880  PMID: 1082135

Abstract

Lymphoid cells from spleen and lymph nodes of BALB/c mice were sensitized in vitro by cocultivation with cells from syngeneic methylcholanthrene-induced sarcomas. The donor mice were either immunized in vivo by tumor transplantation followed by excision ("tumor-excised mice") or were carrying a progressively growing tumor ("tumor-bearing mice"). The cell-mediated immune response of the sensitized cells was compared by a 36-hr microcytotoxicity assay. Lymphoid cells from tumor-excised mice were cytotoxic when tested after 3 days and also after 6 days of sensitization in vitro. Lymphoid cells from tumor-bearing mice were cytotoxic to cells of the tumor borne when tested after 3 days in culture. However, the cytotoxic effect was lost after 6 days of contact with the sensitizing tumor in vitro. Preliminary data suggest that the cultured lymphoid cells of tumor-bearing mice which have lost cytotoxicity, can suppress the reactivity of in vitro sensitized lymphoid cells from tumor-excised mice.

Full text

PDF
5086

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baldwin R. W. Immunological aspects of chemical carcinogenesis. Adv Cancer Res. 1973;18:1–75. doi: 10.1016/s0065-230x(08)60750-2. [DOI] [PubMed] [Google Scholar]
  2. Bansal S. C., Hellström K. E., Hellström I., Sjögren H. O. Cell-mediated immunity and blocking serum activity to tolerated allografts in rats. J Exp Med. 1973 Mar 1;137(3):590–602. doi: 10.1084/jem.137.3.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eggers A. E., Wunderlich J. R. Suppressor cells in tumor-bearing mice capable of nonspecific blocking of in vitro immunization against transplant antigens. J Immunol. 1975 May;114(5):1554–1556. [PubMed] [Google Scholar]
  4. Elkins W. L., Hellström I., Hellström K. E. Transplantation tolerance and enhancement. Concepts and questions. Transplantation. 1974 Jul;18(1):38–45. doi: 10.1097/00007890-197407000-00007. [DOI] [PubMed] [Google Scholar]
  5. Goldmann S. F., Krumbacher K., Schnappauf H., Flad H. D. Definition of MLC specificities in the dog. Transplant Proc. 1975 Sep;7(3):389–393. [PubMed] [Google Scholar]
  6. Howell S. B., Dean J. H., Law L. W. Defects in cell-mediated immunity during growth of a syngeneic simian virus-induced tumor. Int J Cancer. 1975 Jan 15;15(1):152–169. doi: 10.1002/ijc.2910150118. [DOI] [PubMed] [Google Scholar]
  7. Ilfeld D., Carnaud C., Cohen I. R., Trainin N. In vitro cytotoxicity and in vivo tumor enhancement induced by mouse spleen cells autosensitized in vitro. Int J Cancer. 1973 Jul 15;12(1):213–222. doi: 10.1002/ijc.2910120122. [DOI] [PubMed] [Google Scholar]
  8. Jainchill J. L., Todaro G. J. Stimulation of cell growth in vitro by serum with and without growth factor. Relation to contact inhibition and viral transformation. Exp Cell Res. 1970 Jan;59(1):137–146. doi: 10.1016/0014-4827(70)90632-4. [DOI] [PubMed] [Google Scholar]
  9. Kall M. A., Hellström I. Specific stimulatory and cytotoxic effects of lymphocytes sensitized in vitro to either alloantigens or tumor antigens. J Immunol. 1975 Mar;114(3):1083–1088. [PubMed] [Google Scholar]
  10. Medina D., Heppner G. Cell-mediated "immunostimulation" induced by mammary tumour virus-free Balb-c mammary tumours. Nature. 1973 Mar 30;242(5396):329–330. doi: 10.1038/242329a0. [DOI] [PubMed] [Google Scholar]
  11. Plata F., Cerottini J. C., Brunner K. T. Primary and secondary in vitro generation of cytolytic T lymphocytes in the murine sarcoma virus system. Eur J Immunol. 1975 Apr;5(4):227–233. doi: 10.1002/eji.1830050402. [DOI] [PubMed] [Google Scholar]
  12. Prehn R. T., Lappé M. A. An immunostimulation theory of tumor development. Transplant Rev. 1971;7:26–54. doi: 10.1111/j.1600-065x.1971.tb00462.x. [DOI] [PubMed] [Google Scholar]
  13. Wagner H., Röllinghoff M. In vitro induction of tumor-specific immunity. I. Parameters of activation and cytotoxic reactivity of mouse lymphoid cells immunized in vitro against syngeneic and allogeneic plasma cell tumors. J Exp Med. 1973 Jul 1;138(1):1–15. doi: 10.1084/jem.138.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Warnatz H., Scheiffarth F. Cell-mediated immune response of in vitro sensitized lymphocytes to isogeneic methyl-cholanthrene-induced tumor cell lines. Transplantation. 1974 Sep;18(3):273–279. doi: 10.1097/00007890-197409000-00011. [DOI] [PubMed] [Google Scholar]
  15. Warnatz H., Scheiffarth F., Zenker W. Comparative studies on the reactivity of T-cells and non T-cells in lymphocyte transformation experiments and cytotoxicity tests. Cell Immunol. 1975 Jun;17(2):517–524. doi: 10.1016/s0008-8749(75)80055-4. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES