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. 1971 Feb;68(2):271–275. doi: 10.1073/pnas.68.2.271

Calcium as a Regulator of the Proliferation of Normal, but Not of Transformed, Chicken Fibroblasts in a Plasma-Containing Medium

Samuel D Balk 1
PMCID: PMC388915  PMID: 5277067

Abstract

In a culture medium of low calcium concentration containing 5% heat-inactivated chicken plasma, normal chicken fibroblasts divide very slowly, while their counterparts transformed with Schmidt-Ruppin Rous sarcoma virus divide much more rapidly. In the same low-calcium medium, the use of heat-inactivated serum, rather than heat-inactivated plasma, results in rapid division of both normal and transformed cells.

The use of heat-inactivated plasma rather than serum, and the use of a low-calcium medium, combine to permit the demonstration of a marked difference between the proliferative behaviors of normal and neoplastic cells and suggest a basis for the autonomy of the latter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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