Abstract
Bacteriophage Qβ RNA directs the cell-free synthesis of two fMet-containing dipeptides prior to the first round of ribosomal translocation. One of these, fMet-Ala, corresponds to the initial segment of the Qβ coat protein. In the present work we take advantage of the fact that translation of the Qβ RNA polymerase cistron is repressed by its coat protein to correlate the other peptide, fMet-Ser, with the Qβ RNA polymerase gene. Our experiments show that repression affects translation of the first two codons of the polymerase cistron, thereby isolating the effect of Qβ coat repressor. Further studies, using single rounds of translocation of the Qβ mRNA and codon-specific tRNAs, allow us to predict the first three amino acids of the Qβ RNA polymerase protein, fMet-Ser-Lys, and to suggest that the initiation region of the Qβ RNA polymerase cistron has the sequence ···AUG·UC[unk]·AA[unk]···
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Billeter M. A., Dahlberg J. E., Goodman H. M., Hindley J., Weissmann C. Sequence of the first 175 nucleotides from the 5' terminus of Qbeta RNA synthesized in vitro. Nature. 1969 Dec 13;224(5224):1083–1086. doi: 10.1038/2241083a0. [DOI] [PubMed] [Google Scholar]
- De Wachter R., Fiers W. Sequences at the 5'-terminus of bacteriophage Q-beta-RNA. Nature. 1969 Jan 18;221(5177):233–235. doi: 10.1038/221233a0. [DOI] [PubMed] [Google Scholar]
- Eggen K., Nathans D. Regulation of protein synthesis directed by coliphage MS2 RNA. II. In vitro repression by phage coat protein. J Mol Biol. 1969 Jan;39(2):293–305. doi: 10.1016/0022-2836(69)90318-0. [DOI] [PubMed] [Google Scholar]
- Erbe R. W., Nau M. M., Leder P. Translation and translocation of defined RNA messengers. J Mol Biol. 1969 Feb 14;39(3):441–460. doi: 10.1016/0022-2836(69)90137-5. [DOI] [PubMed] [Google Scholar]
- Gesteland R. F., Spahr P. F. Translation of R-17 RNA fragments. Cold Spring Harb Symp Quant Biol. 1969;34:707–716. doi: 10.1101/sqb.1969.034.01.080. [DOI] [PubMed] [Google Scholar]
- Gillam I., Blew D., Warrington R. C., von Tigerstrom M., Tener G. M. A general procedure for the isolation of specific transfer ribonucleic acids. Biochemistry. 1968 Oct;7(10):3459–3468. doi: 10.1021/bi00850a022. [DOI] [PubMed] [Google Scholar]
- Haruna I., Spiegelman S. Specific template requirments of RNA replicases. Proc Natl Acad Sci U S A. 1965 Aug;54(2):579–587. doi: 10.1073/pnas.54.2.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hindley J., Staples D. H. Sequence of a ribosome binding site in bacteriophage Q-beta-RNA. Nature. 1969 Dec 6;224(5223):964–967. doi: 10.1038/224964a0. [DOI] [PubMed] [Google Scholar]
- Konigsberg W., Maita T., Katze J., Weber K. Amino-acid sequence of the "Qbeta" coat protein. Nature. 1970 Jul 18;227(5255):271–273. doi: 10.1038/227271a0. [DOI] [PubMed] [Google Scholar]
- Leder P., Skogerson L. E., Nau M. M. Translocation of mRNA codons. I. The preparation and characteristics of a homogeneous enzyme. Proc Natl Acad Sci U S A. 1969 Feb;62(2):454–460. doi: 10.1073/pnas.62.2.454. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leder P., Skogerson L. E., Roufa D. J. Translocation of mRNA codons. II. Properties of an anti-translocase antibody. Proc Natl Acad Sci U S A. 1969 Mar;62(3):928–933. doi: 10.1073/pnas.62.3.928. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lodish H. F. Bacteriophage f2 RNA: control of translation and gene order. Nature. 1968 Oct 26;220(5165):345–350. doi: 10.1038/220345a0. [DOI] [PubMed] [Google Scholar]
- Lodish H. F., Zinder N. D. Mutants of the bacteriophage f2. 8. Control mechanisms for phage-specific syntheses. J Mol Biol. 1966 Aug;19(2):333–348. doi: 10.1016/s0022-2836(66)80008-6. [DOI] [PubMed] [Google Scholar]
- Lucas-Lenard J., Lipmann F. Separation of three microbial amino acid polymerization factors. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1562–1566. doi: 10.1073/pnas.55.6.1562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NIRENBERG M., LEDER P. RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES. Science. 1964 Sep 25;145(3639):1399–1407. doi: 10.1126/science.145.3639.1399. [DOI] [PubMed] [Google Scholar]
- Nirenberg M., Caskey T., Marshall R., Brimacombe R., Kellogg D., Doctor B., Hatfield D., Levin J., Rottman F., Pestka S. The RNA code and protein synthesis. Cold Spring Harb Symp Quant Biol. 1966;31:11–24. doi: 10.1101/sqb.1966.031.01.008. [DOI] [PubMed] [Google Scholar]
- Robertson H., Webster R. E., Zinder N. D. Bacteriophage coat protein as repressor. Nature. 1968 May 11;218(5141):533–536. doi: 10.1038/218533a0. [DOI] [PubMed] [Google Scholar]
- Roufa D. J., Doctor B. P., Leder P. The two site model of ribosomal function: a test using degenerate serine codons in bacteriophage f2 mRNA. Biochem Biophys Res Commun. 1970 Apr 24;39(2):231–237. doi: 10.1016/0006-291x(70)90783-7. [DOI] [PubMed] [Google Scholar]
- Roufa D. J., Skogerson L. E., Leder P. Translation of phage Qbeta mRNA: a test of the two-site model for ribosomal function. Nature. 1970 Aug 8;227(5258):567–570. doi: 10.1038/227567a0. [DOI] [PubMed] [Google Scholar]
- Steitz J. A. Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA. Nature. 1969 Dec 6;224(5223):957–964. doi: 10.1038/224957a0. [DOI] [PubMed] [Google Scholar]
- Sugiyama T., Hebert R. R., Hartman K. A. Ribonucleoprotein complexes formed between bacteriophage MS2 RNA and MS2 protein in vitro. J Mol Biol. 1967 May 14;25(3):455–463. doi: 10.1016/0022-2836(67)90198-2. [DOI] [PubMed] [Google Scholar]
- Sugiyama T., Nakada D. Control of translation of MS2 RNA cistrons by MS2 coat protein. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1744–1750. doi: 10.1073/pnas.57.6.1744. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Viñuela E., Algranati I. D., Ochoa S. Synthesis of virus-specific proteins in Escherichia coli infected with the RNA bacteriophage MS2. Eur J Biochem. 1967 Mar;1(1):3–11. doi: 10.1007/978-3-662-25813-2_2. [DOI] [PubMed] [Google Scholar]
- Ward R., Shive K., Valentine R. Capsid protein of f2 as translational repressor. Biochem Biophys Res Commun. 1967 Oct 11;29(1):8–13. doi: 10.1016/0006-291x(67)90532-3. [DOI] [PubMed] [Google Scholar]