Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Feb;68(2):287–290. doi: 10.1073/pnas.68.2.287

Defective Production of Mitochondrial Ribosomes in the Poky Mutant of Neurospora crassa

M R Rifkin 1, D J L Luck 1
PMCID: PMC388919  PMID: 5277071

Abstract

A strain of Neurospora crassa containing a cytoplasmic mutation (poky) affecting mitochondrial function is shown to be deficient in small ribosomal subunits in the mitochondrion during the exponential growth phase. In the stationary growth phase, small subunits are more abundant and are present in mitochondrial ribosomal monomers. This change can be correlated with the return of mitochondrial cytochrome content to amounts approaching those of wild type mitochondria. The ribosomal defect shows an extrachromosomal pattern of inheritance in crosses of poky with wild type.

Full text

PDF
287

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARLOW J. J., MATHIAS A. P., WILLIAMSON R., GAMMACK D. B. A SIMPLE METHOD FOR THE QUANTITATIVE ISOLATION OF UNDEGRADED HIGH MOLECULAR WEIGHT RIBONUCLEIC ACID. Biochem Biophys Res Commun. 1963 Sep 10;13:61–66. doi: 10.1016/0006-291x(63)90163-3. [DOI] [PubMed] [Google Scholar]
  2. Küntzel H., Noll H. Mitochondrial and cytoplasmic polysomes from Neurospora crassa. Nature. 1967 Sep 23;215(5108):1340–1345. doi: 10.1038/2151340a0. [DOI] [PubMed] [Google Scholar]
  3. Küntzel H. Proteins of mitochondrial and cytoplasmic ribosomes from Neurospora crassa. Nature. 1969 Apr 12;222(5189):142–146. doi: 10.1038/222142a0. [DOI] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. LUCK D. J. Formation of mitochondria in Neurospora crassa. A quantitative radioautographic study. J Cell Biol. 1963 Mar;16:483–499. doi: 10.1083/jcb.16.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LUCK D. J. THE INFLUENCE OF PRECURSOR POOL SIZE ON MITOCHONDRIAL COMPOSITION IN NEUROSPORA CRASSA. J Cell Biol. 1965 Mar;24:445–460. doi: 10.1083/jcb.24.3.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Linnane A. W., Saunders G. W., Gingold E. B., Lukins H. B. The biogenesis of mitochondria. V. Cytoplasmic inheritance of erythromycin resistance in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1968 Mar;59(3):903–910. doi: 10.1073/pnas.59.3.903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MITCHELL M. B., MITCHELL H. K. A nuclear gene suppressor of a cytoplasmically inherited character in Neurospora crassa. J Gen Microbiol. 1956 Feb;14(1):84–89. doi: 10.1099/00221287-14-1-84. [DOI] [PubMed] [Google Scholar]
  9. Mitchell M. B., Mitchell H. K. A Case of "Maternal" Inheritance in Neurospora Crassa. Proc Natl Acad Sci U S A. 1952 May;38(5):442–449. doi: 10.1073/pnas.38.5.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mitchell M. B., Mitchell H. K., Tissieres A. Mendelian and Non-Mendelian Factors Affecting the Cytochrome System in Neurospora Crassa. Proc Natl Acad Sci U S A. 1953 Jul;39(7):606–613. doi: 10.1073/pnas.39.7.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rifkin M. R., Wood D. D., Luck D. J. Ribosomal RNA and ribosomes from mitochondria of Neurospora crassa. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1025–1032. doi: 10.1073/pnas.58.3.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schmitt H. Characterization of mitochondrial ribosomes from Saccharomyces cerevisiae. FEBS Lett. 1969 Aug;4(3):234–238. doi: 10.1016/0014-5793(69)80243-7. [DOI] [PubMed] [Google Scholar]
  13. Solymosy F., Fedorcsák I., Gulyás A., Farkas G. L., Ehrenberg L. A new method based on the use of diethyl pyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid from plant tissues. Eur J Biochem. 1968 Sep 24;5(4):520–527. doi: 10.1111/j.1432-1033.1968.tb00401.x. [DOI] [PubMed] [Google Scholar]
  14. Stegeman W. J., Cooper C. S., Avers C. J. Physical characterization of ribosomes from purified mitochondria of yeast. Biochem Biophys Res Commun. 1970 Apr 8;39(1):69–76. doi: 10.1016/0006-291x(70)90759-x. [DOI] [PubMed] [Google Scholar]
  15. Swanson R. F., Dawid I. B. The mitochondrial ribosome of Xenopus laevis. Proc Natl Acad Sci U S A. 1970 May;66(1):117–124. doi: 10.1073/pnas.66.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thomas D. Y., Wilkie D. Inhibition of mitochondrial synthesis in yeast by erythromycin: cytoplasmic and nuclear factors controlling resistance. Genet Res. 1968 Feb;11(1):33–41. doi: 10.1017/s0016672300011174. [DOI] [PubMed] [Google Scholar]
  17. Vignais P. V., Huet J., André J. Isolation and characterization of ribosomes from yeast mitochondria. FEBS Lett. 1969 May;3(3):177–181. doi: 10.1016/0014-5793(69)80128-6. [DOI] [PubMed] [Google Scholar]
  18. WILLIAMS J. N., Jr A METHOD FOR THE SIMULTANEOUS QUANTITATIVE ESTIMATION OF CYTOCHROMES A, B, C1, AND C IN MITOCHONDRIA. Arch Biochem Biophys. 1964 Sep;107:537–543. doi: 10.1016/0003-9861(64)90313-3. [DOI] [PubMed] [Google Scholar]
  19. Wood D. D., Luck D. J. Hybridization of mitochondrial ribosomal RNA. J Mol Biol. 1969 Apr;41(2):211–224. doi: 10.1016/0022-2836(69)90386-6. [DOI] [PubMed] [Google Scholar]
  20. Woodward D. O., Munkres K. D. Alterations of a maternally inherited mitochondrial structural protein in respiratory-deficient strains of Neurospora. Proc Natl Acad Sci U S A. 1966 Apr;55(4):872–880. doi: 10.1073/pnas.55.4.872. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES