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. 1971 Feb;68(2):435–439. doi: 10.1073/pnas.68.2.435

Cellular Site of Escherichia coli Ribosomal RNA Synthesis

Anne M Haywood 1
PMCID: PMC388955  PMID: 5277096

Abstract

Escherichia coli, grown for a generation after the addition of [14C]uracil and for one minute after a pulse of [3H]uracil, were lysed in the presence of 6 mM Mg++, and divided into a 30,000 × g “supernatant” and sediment. The sediment was resuspended in buffer containing no divalent cations, and again centrifuged to give the “Mg++ sediment” and the remaining “sediment”. The ratio of 3H (one-minute pulse RNA) to 14C (Stable RNA) in the supernatant is 1/6 to 1/10 that of the sediment RNA, and the ratio of 3H:14C in the Mg++ sediment is 1/2 to 1/6 that in the sediment RNA. Gel electrophoresis of RNA from the different fractions shows that ribosomal RNA precursors first appear in the sediment. It is concluded that the site of ribosomal RNA synthesis is associated with a sedimentable structure, which is probably cell membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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