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. 1971 Feb;68(2):473–477. doi: 10.1073/pnas.68.2.473

DNA-Dependent RNA Polymerase from Yeast Mitochondria

Ming-Jer Tsai 1, Georg Michaelis 1, R S Criddle 1
PMCID: PMC388963  PMID: 5277104

Abstract

The DNA-dependent RNA polymerase present in the mitochondria of Saccharomyces cerevisiae has been solubilized using a 0.5 M KCI solution, and the soluble enzyme has been purified. Two forms of mitochondrial enzyme were obtained; they differ in their template specificity and metal-ion dependency. The mitochondrial RNA polymerase also differs from enzyme obtained from the nucleus with respect to antibiotic sensitivity and template specificity. Only the nuclear enzyme is sensitive to α-amanitin inhibition. The relative activities of the isolated nuclear and mitochondrial polymerases toward their homologous DNAs are consistent with their in vivo functions. The possibilities of bacterial- and nuclear-enzyme contamination of the mitochondrial enzyme preparation have been ruled out. RNA polymerase activity in two petite mutants has been studied. Isolated mitochondria from a petite mutant with no detectable mitochondrial DNA has greatly diminished mitochondrial DNA-dependent RNA polymerase activity, while a petite mutant with only a small change in mitochondrial DNA base composition has normal amounts of enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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