Table 1.
Presence or absence of lentiviral vector and/or green fluorescent protein (GFP) DNA in the periodic semen samples and the testes samples collected at the end of the study.
| Treatment | Monkey Number |
Sperm from Semen | Testes |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sham | Trans- planted |
||||||||||||
| Pre- Irrad |
Time after Irradiation (weeks)a |
||||||||||||
| 16 | 20 | 24 | 28 | 32 | 36 | 40 | 44 | 44 | |||||
| Irradiated | 1 | − | 0 | 0 | 0 | n | 0 | n | − | n | nd | nd | |
| 2 | nd | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | − | − | ||
| 3 | nd | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | nd | nd | ||
| 4 | − | 0 | 0 | nd | n | 0 | 0 | 0 | 0 | nd | nd | ||
| 5 | nd | 0 | nd | nd | 0 | nd | − | − | 0 | nd | nd | ||
| 6 | − | + | − | − | − | − | + | − | + | −b | −b | ||
| Irradiated + GnRH-ant |
7 | − | 0 | − | − | − | − | + | − | + | −b | −b | |
| 8 | − | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | nd | nd | ||
| 9 | − | 0 | + | − | − | + | + | − | ne | − | − | ||
| 10 | − | 0 | 0 | − | − | − | + | − | + | − | − | ||
| 11 | − | 0 | − | nd | n | − | n | ne | n | − | + | ||
| 12 | − | 0 | ne | n | + | nd | + | n | − | − | − | ||
Abbreviations: n = no ejaculate; ne = not enough ejaculate for analysis; 0 = azoospermic; + = presence of lentiviral vector and/or GFP DNA in the semen sample; − =absence of lentiviral vector and/or GFP DNA in the semen sample; nd = not done.
a
Note transplantation was done 8 weeks after irradiation
b
Low numbers of germ cells in testes (see Fig. 4B).