Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Mar;68(3):580–584. doi: 10.1073/pnas.68.3.580

Use of Monkey-Mouse Hybrid Cells for the Study of the Cellular Regulation of Interferon Production and Action

R Cassingena 1,2,3, C Chany 1,2,3, M Vignal 1,2,3, H Suarez 1,2,3, S Estrade 1,2,3, P Lazar 1,2,3
PMCID: PMC388994  PMID: 5276765

Abstract

Four clones of a somatic monkey-mouse hybrid cell line were studied. One of these clones produced both mouse and monkey interferon, while the three others produced only mouse interferon. All four were, however, sensitive to both mouse and monkey (or human) interferon. The karyotype analysis of these four hybrid clones and the parental cell lines enabled us to locate the possible genetic site governing the synthesis of monkey interferon on a small subtelocentric monkey chromosome. The genetic site responsible for the synthesis of the antiviral protein is located on a different (monkey or mouse) chromosome.

Full text

PDF
580

Images in this article

582Image
on p.582
582Image
on p.582
582Image
on p.582

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHANY C. An interferon-like inhibitor of viral multiplication from malignant cells (the viral autoinhibition phenomenon). Virology. 1961 Apr;13:485–492. doi: 10.1016/0042-6822(61)90279-3. [DOI] [PubMed] [Google Scholar]
  2. Cassingena R., Chany C., Vignal M., Estrade S., Suarez H. G. Utilisation de cellules hybrides (singe-souris) pour l'étude de la régulation cellulaire de la production et de l'action de l'interférone. C R Acad Sci Hebd Seances Acad Sci D. 1970 Feb 23;270(8):1189–1191. [PubMed] [Google Scholar]
  3. Chany C., Vignal M. Etude du mécanisme de l'étst réfractaiere des cellules à l1 production d'interféron, près inductions répétées. C R Acad Sci Hebd Seances Acad Sci D. 1968 Nov 18;267(21):1798–1800. [PubMed] [Google Scholar]
  4. Dubbs D. R., Kit S., De Torres R. A., Anken M. Virogenic properties of bromodeoxyuridine-sensitive and bromodeoxyuridine-resistant simian virus 40-transformed mouse kidney cells. J Virol. 1967 Oct;1(5):968–979. doi: 10.1128/jvi.1.5.968-979.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GRESSER I. Production of interferon by suspensions of human leucocytes. Proc Soc Exp Biol Med. 1961 Dec;108:799–803. doi: 10.3181/00379727-108-27072. [DOI] [PubMed] [Google Scholar]
  6. Guggenheim M. A., Friedman R. M., Rabson A. S. Interferon action in heterokaryons. Proc Soc Exp Biol Med. 1969 Apr;130(4):1242–1245. doi: 10.3181/00379727-130-33763. [DOI] [PubMed] [Google Scholar]
  7. JENSEN F. C., GIRARDI A. J., GILDEN R. V., KOPROWSKI H. INFECTION OF HUMAN AND SIMIAN TISSUE CULTURES WITH ROUS SARCOMA VIRUS. Proc Natl Acad Sci U S A. 1964 Jul;52:53–59. doi: 10.1073/pnas.52.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kit S., Nakajima K., Kurimura T., Dubbs D. R., Cassingena R. Monkey-mouse hybrid cell lines containing the SV40 genome in a partially repressed state. Int J Cancer. 1970 Jan 15;5(1):1–14. doi: 10.1002/ijc.2910050102. [DOI] [PubMed] [Google Scholar]
  9. LITTLEFIELD J. W. STUDIES ON THYMIDINE KINASE IN CULTURED MOUSE FIBROBLASTS. Biochim Biophys Acta. 1965 Jan 11;95:14–22. doi: 10.1016/0005-2787(65)90206-6. [DOI] [PubMed] [Google Scholar]
  10. MONTAGNIER L., MACPHERSON I. CROISSANCE S'ELECTIVE EN G'ELOSE DE CELLULES DE HAMSTER TRANSFORM'EES PAR LE VIRUS DU POLYOME. C R Hebd Seances Acad Sci. 1964 Apr 20;258:4171–4173. [PubMed] [Google Scholar]
  11. TYRRELL D. A. Interferon produced by cultures of calf kidney cells. Nature. 1959 Aug 8;184(Suppl 7):452–453. doi: 10.1038/184452a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES