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. 1971 Mar;68(3):605–608. doi: 10.1073/pnas.68.3.605

Inhibition of the Metabolism of 25-Hydroxycholecalciferol by Actinomycin D and Cycloheximide

Y Tanaka 1, H F Deluca 1
PMCID: PMC388999  PMID: 4322523

Abstract

Actinomycin D (or cycloheximide) administered prior to radioactive 25-hydroxycholecalciferol blocks the metabolism of 25-hydroxycholecalciferol to polar metabolites that accumulate in intestinal tissue, while it does not prevent the 25-hydroxylation of vitamin D3 in the liver. Actinomycin D given after radioactive 25-hydroxycholecalciferol does not inhibit 25-hydroxycholecalciferol metabolism. These results indicate that 25-hydroxycholecalciferol must interact with the nuclei of cells to bring about the production of an enzyme(s) that converts it to its polar metabolites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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