Abstract
Erythropoietin, the hormone that is the primary regulator of erythrocyte production, has been purified from anemic-sheep plasma, with a purification factor of more than 1 million. Gel electrophoresis shows that the major contaminant is desialated erythropoietin.
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- Dunker A. K., Rueckert R. R. Observations on molecular weight determinations on polyacrylamide gel. J Biol Chem. 1969 Sep 25;244(18):5074–5080. [PubMed] [Google Scholar]
- FRIED W., GOLDWASSER E., JACOBSON L. O., PLZAK L. F. Studies on erythropoiesis. III. Factors controlling erythropoietin production. Proc Soc Exp Biol Med. 1957 Jan;94(1):237–241. doi: 10.3181/00379727-94-22910. [DOI] [PubMed] [Google Scholar]
- GOLDWASSER E., WHITE W. F., TAYLOR K. B. Further purification of sheep plasma erythropoietin. Biochim Biophys Acta. 1962 Nov 5;64:487–496. doi: 10.1016/0006-3002(62)90306-2. [DOI] [PubMed] [Google Scholar]
- Goldwasser E., Kung C. K. Progress in the purfication of erythropoietin. Ann N Y Acad Sci. 1968 Mar 29;149(1):49–53. doi: 10.1111/j.1749-6632.1968.tb15135.x. [DOI] [PubMed] [Google Scholar]
- JACOBSON L. O., GOLDWASSER E., PLZAK L. F., FRIED W. Studies on erythropoiesis. IV. Reticulocyte response of hypophysectomized and polycythemic rodents to erythropoietin. Proc Soc Exp Biol Med. 1957 Feb;94(2):243–249. doi: 10.3181/00379727-94-22911. [DOI] [PubMed] [Google Scholar]
- Lowy P. H., Keighley G. L. Extraction of erythropoietin from human urine and rabbit plasma. Clin Chim Acta. 1966 Apr;13(4):491–497. doi: 10.1016/0009-8981(66)90241-5. [DOI] [PubMed] [Google Scholar]
- MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
- McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]