Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Apr;68(4):731–735. doi: 10.1073/pnas.68.4.731

Interconversion of Cyclic Nucleotide-Activated and Cyclic Nucleotide-Independent Forms of a Protein Kinase from Beef Heart

Jack Erlichman 1,2, Allen H Hirsch 1,2, Ora M Rosen 1,2,*
PMCID: PMC389030  PMID: 4323785

Abstract

A protein kinase activated by cyclic nucleotides was purified from beef heart. Upon exposure to adenosine 3′:5′-cyclic monophosphate (cyclic AMP) during gel filtration on Sephadex G-200, the protein kinase dissociated into a cyclic nucleotide-independent protein kinase and a cyclic nucleotide-binding protein. A similar or identical cyclic nucleotide-independent protein kinase could be obtained in highly purified form by clution from a DEAE-cellulose column with 10-6 M cyclic AMP; the cyclic AMP-binding protein was apparently retained by the resin. The addition of cyclic nucleotide-binding protein to cyclic nucleotide-independent protein kinase resulted in the reappearance of cyclic nucleotide-dependent protein kinase, which could be isolated by filtration on Sephadex G-200 in the absence of cyclic AMP. These results confirm and extend previous suggestions that cyclic nucleotides activate protein kinases by dissociating them from inhibitory, cyclic nucleotide-binding proteins.

Full text

PDF
731

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Corbin J. D., Krebs E. G. A cyclic AMP--stimulated protein kinase in adipose tissue. Biochem Biophys Res Commun. 1969 Jul 23;36(2):328–336. doi: 10.1016/0006-291x(69)90334-9. [DOI] [PubMed] [Google Scholar]
  3. Corbin J. D., Reimann E. M., Walsh D. A., Krebs E. G. Activation of adipose tissue lipase by skeletal muscle cyclic adenosine 3',5'- monophosphate-stimulated protein kinase. J Biol Chem. 1970 Sep 25;245(18):4849–4851. [PubMed] [Google Scholar]
  4. DeLange R. J., Kemp R. G., Riley W. D., Cooper R. A., Krebs E. G. Activation of skeletal muscle phosphorylase kinase by adenosine triphosphate and adenosine 3',5'-monophosphate. J Biol Chem. 1968 May 10;243(9):2200–2208. [PubMed] [Google Scholar]
  5. Gill G. N., Garren L. D. A cyclic-3',5'-adenosine monophosphate dependent protein kinase from the adrenal cortex: comparison with a cyclic AMP binding protein. Biochem Biophys Res Commun. 1970 May 11;39(3):335–343. doi: 10.1016/0006-291x(70)90581-4. [DOI] [PubMed] [Google Scholar]
  6. Gill G. N., Garren L. D. On the mechanism of action of adrenocorticotropic hormone: the binding of cyclic-3',5'-adenosine monophosphate to an adrenal cortical protein. Proc Natl Acad Sci U S A. 1969 Jun;63(2):512–519. doi: 10.1073/pnas.63.2.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goodman D. B., Rasmussen H., DiBella F., Guthrow C. E., Jr Cyclic adenosine 3':5'-monophosphate-stimulated phosphorylation of isolated neurotubule subunits. Proc Natl Acad Sci U S A. 1970 Oct;67(2):652–659. doi: 10.1073/pnas.67.2.652. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Huttunen J. K., Steinberg D., Mayer S. E. ATP-dependent and cyclic AMP-dependent activation of rat adipose tissue lipase by protein kinase from rabbit skeletal muscle. Proc Natl Acad Sci U S A. 1970 Sep;67(1):290–295. doi: 10.1073/pnas.67.1.290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kumon A., Yamamura H., Nishizuka Y. Mode of action of adenosine 3',5'-cyclic phosphate on protein kinase from rat liver. Biochem Biophys Res Commun. 1970 Dec 9;41(5):1290–1297. doi: 10.1016/0006-291x(70)90228-7. [DOI] [PubMed] [Google Scholar]
  11. Kuo J. F., Greengard P. An adenosine 3',5'-monophosphate-dependent protein kinase from Escherichia coli. J Biol Chem. 1969 Jun 25;244(12):3417–3419. [PubMed] [Google Scholar]
  12. Kuo J. F., Greengard P. Cyclic nucleotide-dependent protein kinases. IV. Widespread occurrence of adenosine 3',5'-monophosphate-dependent protein kinase in various tissues and phyla of the animal kingdom. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1349–1355. doi: 10.1073/pnas.64.4.1349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Langan T. A. Action of adenosine 3',5'-monophosphate-dependent histone kinase in vivo. J Biol Chem. 1969 Oct 25;244(20):5763–5765. [PubMed] [Google Scholar]
  15. Langan T. A. Histone phosphorylation: stimulation by adenosine 3',5'-monophosphate. Science. 1968 Nov 1;162(3853):579–580. doi: 10.1126/science.162.3853.579. [DOI] [PubMed] [Google Scholar]
  16. Miyamoto E., Kuo J. F., Greengard P. Adenosine 3',5'-monophosphate-dependent protein kinase from brain. Science. 1969 Jul 4;165(3888):63–65. doi: 10.1126/science.165.3888.63. [DOI] [PubMed] [Google Scholar]
  17. Reimann E. M., Brostrom C. O., Corbin J. D., King C. A., Krebs E. G. Separation of regulatory and catalytic subunits of the cyclic 3',5'-adenosine monophosphate-dependent protein kinase(s) of rabbit skeletal muscle. Biochem Biophys Res Commun. 1971 Jan 22;42(2):187–194. doi: 10.1016/0006-291x(71)90086-6. [DOI] [PubMed] [Google Scholar]
  18. Robison G. A., Butcher R. W., Sutherland E. W. Cyclic AMP. Annu Rev Biochem. 1968;37:149–174. doi: 10.1146/annurev.bi.37.070168.001053. [DOI] [PubMed] [Google Scholar]
  19. Rosen O. M. Preparation and properties of a cyclic 3',5'-nucleotide phosphodiesterase isolated from frog erythrocytes. Arch Biochem Biophys. 1970 Apr;137(2):435–441. doi: 10.1016/0003-9861(70)90460-1. [DOI] [PubMed] [Google Scholar]
  20. Tao M., Salas M. L., Lipmann F. Mechanism of activation by adenosine 3':5'-cyclic monophosphate of a protein phosphokinase from rabbit reticulocytes. Proc Natl Acad Sci U S A. 1970 Sep;67(1):408–414. doi: 10.1073/pnas.67.1.408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Walsh D. A., Perkins J. P., Krebs E. G. An adenosine 3',5'-monophosphate-dependant protein kinase from rabbit skeletal muscle. J Biol Chem. 1968 Jul 10;243(13):3763–3765. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES