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. 1971 Apr;68(4):736–739. doi: 10.1073/pnas.68.4.736

A Simple, Sensitive Protein-binding Assay for Guanosine 3′:5′-Monophosphate

Ferid Murad *, Vincent Manganiello †, Martha Vaughan †,‡
PMCID: PMC389031  PMID: 4323786

Abstract

A protein-binding assay for guanosine 3′:5′-monophosphate, which employs an enzyme preparation from lobster tail muscle, is described. The binding constant for cyclic GMP was 2-10 nM for four different enzyme preparations. The method can be set up in several days and permits the detection of as little as 0.5-1 pmol of cyclic GMP. In some instances, it is possible to assay the nucleotide in urine or deproteinized extracts of tissue without purification. For other samples, a simple method for separating and purifying cyclic AMP and cyclic GMP is described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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