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. 1971 Apr;68(4):761–764. doi: 10.1073/pnas.68.4.761

Purification and DNA Synthesis in Cell-Free Extracts: Properties of DNA Polymerase II

Thomas Kornberg 1, Malcolm L Gefter 1
PMCID: PMC389037  PMID: 4927672

Abstract

The major DNA-synthesizing enzyme present in Pol A1-Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg++, NH4+, and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A1- extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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