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. 1971 May;68(5):880–884. doi: 10.1073/pnas.68.5.880

Phosphorylation of Ribosome-Associated Protein by an Adenosine 3′:5′-Cyclic Monophosphate-Dependent Protein Kinase: Location of the Microsomal Receptor and Protein Kinase

Gordon M Walton 1, Gordon N Gill 1, Itamar B Abrass 1, Leonard D Garren 1
PMCID: PMC389071  PMID: 4325002

Abstract

In the adrenal cortex, the adenosine 3′:5′-cyclic monophosphate (cAMP)-receptor protein and the cAMP-dependent protein kinase are located in both the cytosol and endoplasmic reticulum. The cAMP-dependent protein kinase from the cytosol catalyzes the phosphorylation of ribosome-associated protein. Ribosomes washed in 0.5 M NH4Cl retain the substrate of the protein kinase reaction, but are dependent on the NH4Cl extract for in vitro protein synthesis. Dissociation of the 80S ribosomes by 0.88 M KCl, however, releases the ribosome-associated (protein) substrate of the phosphorylation reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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