Abstract
Adenylate cyclase of homogenates or lysates of mouse and rat lymphoid tissues was activated by the addition of fluoride ion, epinephrine, or norepinephrine, but not by any of several other hormones. The catecholamine stimulation was characterized as β-adrenergic. This activity was localized in the small lymphoid cells, was greater in thymic than in splenic or mesenteric node cells, and also was greater in mouse than in rat cells. Catecholamine-stimulated activity of mouse thymocytes remained constant from the 17-19th day of fetal development to 5 weeks after birth; it subsequently decreased to about one-half of the activity by 7-8 weeks. Similar decreases with age occurred in mouse spleen and rat thymus. In contrast, the glucagon-stimulated activity of rat liver increased during a similar period. Hypophysectomy of rats at 3 weeks did not influence the amount of cyclase activity of thymocytes assayed at 7 weeks.
When intact mouse thymocytes were first incubated in a culture medium at 37°C with epinephrine or norepinephrine, a second addition of catecholamine after cell lysis no longer stimulated the enzymes. This loss of stimulation was selective, since basal activity and stimulation by fluoride ion were not affected. Incubation of intact cells with phytohemagglutinin markedly decreased the activity of lysates, whether assayed in the presence or absence of catecholamine or fluoride. In contrast, phytohemagglutinin added directly to the assay had no effect. No alternations occurred in adenylate cyclase activity as a result of the incubation of a 1:1 mixture of thymocytes from two strains of mice selected for the capacity of the cells to produce a mixed lymphocyte response.
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Selected References
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- Cohen J. J., Fschbach M., Claman H. N. Hydrocortisne resistance of graft vs host activity in mouse thymus, spleen and bone marrow. J Immunol. 1970 Nov;105(5):1146–1150. [PubMed] [Google Scholar]
- Dicke K. A., Tridente G., van Bekkum D. W. The selective elimination of immunologically competent cells from bone marrow and lymphocyte cell mixtures. 3. In vitro test for detection of immunocompetent cells in fractionated mouse spleen cell suspensions and primate bone marrow suspensions. Transplantation. 1969 Oct;8(4):422–434. doi: 10.1097/00007890-196910000-00014. [DOI] [PubMed] [Google Scholar]
- EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
- Grahame-Smith D. G., Butcher R. W., Ney R. L., Sutherland E. W. Adenosine 3',5'-monophosphate as the intracellular mediator of the action of adrenocorticotropic hormone on the adrenal cortex. J Biol Chem. 1967 Dec 10;242(23):5535–5541. [PubMed] [Google Scholar]
- Hedeskov C. J. Early effects of phytohaemagglutinin on glucose metabolism of normal human lymphocytes. Biochem J. 1968 Nov;110(2):373–380. doi: 10.1042/bj1100373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirschhorn R., Grossman J., Weissmann G. Effect of cyclic 3',5'-adenosine monophosphate and theophylline on lymphocyte transformation. Proc Soc Exp Biol Med. 1970 Apr;133(4):1361–1365. doi: 10.3181/00379727-133-34690. [DOI] [PubMed] [Google Scholar]
- Ishikawa E., Ishikawa S., Davis J. W., Sutherland E. W. Determination of guanosine 3',5'-monophosphate in tissues and of guanyl cyclase in rat intestine. J Biol Chem. 1969 Dec 10;244(23):6371–6376. [PubMed] [Google Scholar]
- Ishizuka M., Gafni M., Braun W. Cyclic AMP effects on antibody formation and their similarities to hormone-mediated events. Proc Soc Exp Biol Med. 1970 Sep;134(4):963–967. doi: 10.3181/00379727-134-34921. [DOI] [PubMed] [Google Scholar]
- KLAINER L. M., CHI Y. M., FREIDBERG S. L., RALL T. W., SUTHERLAND E. W. Adenyl cyclase. IV. The effects of neurohormones on the formation of adenosine 3',5'-phosphate by preparations from brain and other tissues. J Biol Chem. 1962 Apr;237:1239–1243. [PubMed] [Google Scholar]
- Kakiuchi S., Rall T. W. Studies on adenosine 3',5'-phosphate in rabbit cerebral cortex. Mol Pharmacol. 1968 Jul;4(4):379–388. [PubMed] [Google Scholar]
- Macmanus J. P., Whitfield J. F. Inhibition by thyrocalcitonin of the mitogenic actions of parathyroid hormone and cyclic adenosine=3',5'-monophosphate on rat thymocytes. Endocrinology. 1970 Apr;86(4):934–939. doi: 10.1210/endo-86-4-934. [DOI] [PubMed] [Google Scholar]
- Macmanus J. P., Whitfield J. F. Stimulation of DNA synthesis and mitotic activity of thymic lymphocytes by cyclic adenosine 3'5'-monophosphate. Exp Cell Res. 1969 Nov;58(1):188–191. doi: 10.1016/0014-4827(69)90135-9. [DOI] [PubMed] [Google Scholar]
- Makman M. H. Adenyl cyclase of cultured mammalian cells: activation by catecholamines. Science. 1970 Dec 25;170(3965):1421–1423. doi: 10.1126/science.170.3965.1421. [DOI] [PubMed] [Google Scholar]
- Makman M. H., Dvorkin B., White A. Influence of cortisol on the utilization of precursors of nucleic acids and protein by lymphoid cells in vitro. J Biol Chem. 1968 Apr 10;243(7):1485–1497. [PubMed] [Google Scholar]
- Miller J. F., Osoba D. Current concepts of the immunological function of the thymus. Physiol Rev. 1967 Jul;47(3):437–520. doi: 10.1152/physrev.1967.47.3.437. [DOI] [PubMed] [Google Scholar]
- Novogrodsky A., Katchalski E. Effect of phytohemagglutinin and prostaglandins on cyclic AMP synthesis in rat lymph node lymphocytes. Biochim Biophys Acta. 1970 Aug 14;215(2):291–296. doi: 10.1016/0304-4165(70)90027-9. [DOI] [PubMed] [Google Scholar]