Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 May;68(5):925–929. doi: 10.1073/pnas.68.5.925

Localization of a Portion of the Ribosomal RNA Genes in Escherichia coli

Linda S Birnbaum 1, Sam Kaplan 1
PMCID: PMC389082  PMID: 4930242

Abstract

About one-half of the genes coding for ribosomal RNA in Escherichia coli have been localized by the use of F-merogenotes. These cistrons have been positioned between 74 and 77 min on the E. coli map described by Taylor and Trotter. The episomal DNA carrying these genes was enriched by chromatography. The map position is discussed with reference to previous localization attempts.

Full text

PDF
925

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADELBERG E. A., BURNS S. N. Genetic variation in the sex factor of Escherichia coli. J Bacteriol. 1960 Mar;79:321–330. doi: 10.1128/jb.79.3.321-330.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Attardi G., Amaldi F. Structure and synthesis of ribosomal RNA. Annu Rev Biochem. 1970;39:183–226. doi: 10.1146/annurev.bi.39.070170.001151. [DOI] [PubMed] [Google Scholar]
  3. BERNS K. I., THOMAS C. A., Jr ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE. J Mol Biol. 1965 Mar;11:476–490. doi: 10.1016/s0022-2836(65)80004-3. [DOI] [PubMed] [Google Scholar]
  4. BROWN D. D., GURDON J. B. ABSENCE OF RIBOSOMAL RNA SYNTHESIS IN THE ANUCLEOLATE MUTANT OF XENOPUS LAEVIS. Proc Natl Acad Sci U S A. 1964 Jan;51:139–146. doi: 10.1073/pnas.51.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  6. CERIOTTI G. A microchemical determination of desoxyribonucleic acid. J Biol Chem. 1952 Sep;198(1):297–303. [PubMed] [Google Scholar]
  7. Caro L., Berg C. M. Chromosome replication in Escherichia coli. II. Origin of replication in F- and F+ strains. J Mol Biol. 1969 Oct 28;45(2):325–336. doi: 10.1016/0022-2836(69)90108-9. [DOI] [PubMed] [Google Scholar]
  8. Colli W., Oishi M. Ribosomal RNA genes in bacteria: evidence for the nature of the physical linkage between 16S and 23S RNA genes in Bacillus subtilis. Proc Natl Acad Sci U S A. 1969 Oct;64(2):642–649. doi: 10.1073/pnas.64.2.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cutler R. G., Evans J. E. Relative transcription activity of different segments of the genome throughout the cell division cycle of Escherichia coli. The mapping of ribosomal and transfer RNA and the determination of the direction of replication. J Mol Biol. 1967 May 28;26(1):91–105. doi: 10.1016/0022-2836(67)90263-x. [DOI] [PubMed] [Google Scholar]
  10. Dubnau D., Smith I., Marmur J. Gene conservation in Bacillus species. II. The location of genes concerned with the synthesis of ribosomal components and soluble RNA. Proc Natl Acad Sci U S A. 1965 Sep;54(3):724–730. doi: 10.1073/pnas.54.3.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. FALKOW S., WOHLHIETER J. A., CITARELLA R. V., BARON L. S. TRANSFER OF EPISOMIC ELEMENTS TO PROTEUS. I. TRANSFER OF F-LINKED CHROMOSOMAL DETERMINANTS. J Bacteriol. 1964 Jan;87:209–219. doi: 10.1128/jb.87.1.209-219.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gillam I., Millward S., Blew D., von Tigerstrom M., Wimmer E., Tener G. M. The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose. Biochemistry. 1967 Oct;6(10):3043–3056. doi: 10.1021/bi00862a011. [DOI] [PubMed] [Google Scholar]
  13. Goldthwaite C., Dubnau D., Smith I. Genetic mapping of antibiotic resistance in markers Bacillus subtilis. Proc Natl Acad Sci U S A. 1970 Jan;65(1):96–103. doi: 10.1073/pnas.65.1.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kaplan S., Anderson D. Selection of temperature-sensitive activating enzyme mutants in Escherichia coli. J Bacteriol. 1968 Mar;95(3):991–997. doi: 10.1128/jb.95.3.991-997.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
  18. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  19. Miller L., Knowland J. Reduction of ribosomal RNA synthesis and ribosomal RNA genes in a mutant of Xenopus laevis which organizes only a partial nucleolus. II. The number of ribosomal RNA genes in animals of different nucleolar types. J Mol Biol. 1970 Nov 14;53(3):329–338. doi: 10.1016/0022-2836(70)90069-0. [DOI] [PubMed] [Google Scholar]
  20. Novick R. P. Extrachromosomal inheritance in bacteria. Bacteriol Rev. 1969 Jun;33(2):210–263. doi: 10.1128/br.33.2.210-263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Purdom I., Bishop J. O., Birnstiel M. L. Scattered arrangement of the bacterial ribosomal cistrons. Nature. 1970 Jul 18;227(5255):239–242. doi: 10.1038/227239a0. [DOI] [PubMed] [Google Scholar]
  22. RITOSSA F. M., SPIEGELMAN S. LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER. Proc Natl Acad Sci U S A. 1965 Apr;53:737–745. doi: 10.1073/pnas.53.4.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rownd R., Nakaya R., Nakamura A. Molecular nature of the drug-resistance factors of the Enterobacteriaceae. J Mol Biol. 1966 Jun;17(2):376–393. doi: 10.1016/s0022-2836(66)80149-3. [DOI] [PubMed] [Google Scholar]
  24. Rownd R. Replication of a bacterial episome under relaxed control. J Mol Biol. 1969 Sep 28;44(3):387–402. doi: 10.1016/0022-2836(69)90368-4. [DOI] [PubMed] [Google Scholar]
  25. Rudner R., Rejman E., Chargaff E. Genetic implications of periodic pulsations of the rate of synthesis and the composition of rapidly labeled bacterial RNA. Proc Natl Acad Sci U S A. 1965 Sep;54(3):904–911. doi: 10.1073/pnas.54.3.904. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  27. Sedat J. W., Kelly R. B., Sinsheimer R. L. Fractionation of nucleic acid on benzoylated-naphthoylated DEAE cellulose. J Mol Biol. 1967 Jun 28;26(3):537–540. doi: 10.1016/0022-2836(67)90321-x. [DOI] [PubMed] [Google Scholar]
  28. Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Ward C. B., Glaser D. A. Origin and direction of DNA synthesis in E. coli B-r. Proc Natl Acad Sci U S A. 1969 Mar;62(3):881–886. doi: 10.1073/pnas.62.3.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wolf B., Newman A., Glaser D. A. On the origin and direction of replication of the Escherichia coli K12 chromosome. J Mol Biol. 1968 Mar 28;32(3):611–629. doi: 10.1016/0022-2836(68)90346-x. [DOI] [PubMed] [Google Scholar]
  31. Yu M. T., Vermeulen C. W., Atwood K. C. Location of the genes for 16S and 23S ribosomal RNA in the genetic map of Escherichia coli. Proc Natl Acad Sci U S A. 1970 Sep;67(1):26–31. doi: 10.1073/pnas.67.1.26. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES