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. 1971 May;68(5):964–968. doi: 10.1073/pnas.68.5.964

Specific Inactivation of 16S Ribosomal RNA Induced by Colicin E3 In Vivo*

C M Bowman *,†,‡, J E Dahlberg †, T Ikemura †, J Konisky *,†,‡,‡, M Nomura *,†,‡
PMCID: PMC389091  PMID: 4930244

Abstract

Treatment of sensitive Escherichia coli cells with colicin E3 leads to inactivation of 30S ribosomal subunits. In vitro reconstitution of 30S subunits indicates that the E3-induced defect lies solely in the 16S RNA. 16S RNA from E3-treated cells lacks several T1 RNase oligonucleotides of normal 16S RNA, including the one from the 3′-end of the 16S RNA, as analyzed by the fingerprint technique of Sanger. An RNA fragment about 50 nucleotides long has been isolated from E3-treated cells. This RNA contains the original 3′-terminal oligonucleotide and other oligonucleotides missing in the E3-16S RNA. The results show that colicin E3 treatment causes the cleavage of 16S RNA at a specific position near the 3′-terminus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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