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. 1971 May;68(5):997–1001. doi: 10.1073/pnas.68.5.997

Analysis of Enzyme Variation in Natural Populations of the Butterfly Colias eurytheme

George B Johnson 1
PMCID: PMC389099  PMID: 5280534

Abstract

The use of internal standards in the separation of proteins by gel electrophoresis is shown to offer significant advantages over other control procedures heretofore used in electrophoretic surveys of enzyme variation in natural populations. By the use of such internal standards, a previously undetected class of micro-variability among the enzymes of wild insects has been demonstrated.

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Selected References

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  1. BERSON S. A., YALLOW R. S. Immunochemical distinction between insulins with identical amino-acid sequences. Nature. 1961 Sep 30;191:1392–1393. doi: 10.1038/1911392a0. [DOI] [PubMed] [Google Scholar]
  2. Brentani R., Brentani M. Messenger ribonucleic acid in the nucleolus. Genetics. 1969;61(1 Suppl):391–399. [PMC free article] [PubMed] [Google Scholar]
  3. Burns J. M., Johnson F. M. Esterase polymorphism in natural populations of a sulfur butterfly, Colias eurytheme. Science. 1967 Apr 7;156(3771):93–96. doi: 10.1126/science.156.3771.93. [DOI] [PubMed] [Google Scholar]
  4. Cann J. R. Multiple electrophoretic zones arising from protein-buffer interaction. Biochemistry. 1966 Mar;5(3):1108–1112. doi: 10.1021/bi00867a042. [DOI] [PubMed] [Google Scholar]
  5. Chen S. H., Sutton H. E. Bovine transferrins: sialic acid and the complex phenotype. Genetics. 1967 Jul;56(3):425–430. doi: 10.1093/genetics/56.3.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  7. Efron Y. Tissue specific variation in the isozyme pattern of the ap( 1) Acid phosphatase in maize. Genetics. 1970 Aug;65(4):575–583. doi: 10.1093/genetics/65.4.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Henderson N. S. Intracellular location and genetic control of isozymes of NADP-dependent isocitrate dehydrogenase and malate dehydrogenase. Ann N Y Acad Sci. 1968 Jun 14;151(1):429–440. doi: 10.1111/j.1749-6632.1968.tb11906.x. [DOI] [PubMed] [Google Scholar]
  9. Hubby J. L., Lewontin R. C. A molecular approach to the study of genic heterozygosity in natural populations. I. The number of alleles at different loci in Drosophila pseudoobscura. Genetics. 1966 Aug;54(2):577–594. doi: 10.1093/genetics/54.2.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kitto G. B., Wassarman P. M., Kaplan N. O. Enzymatically active conformers of mitochondrial malate dehydrogenase. Proc Natl Acad Sci U S A. 1966 Aug;56(2):578–585. doi: 10.1073/pnas.56.2.578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lewontin R. C., Hubby J. L. A molecular approach to the study of genic heterozygosity in natural populations. II. Amount of variation and degree of heterozygosity in natural populations of Drosophila pseudoobscura. Genetics. 1966 Aug;54(2):595–609. doi: 10.1093/genetics/54.2.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Prakash S., Lewontin R. C., Hubby J. L. A molecular approach to the study of genic heterozygosity in natural populations. IV. Patterns of genic variation in central, marginal and isolated populations of Drosophila pseudoobscura. Genetics. 1969 Apr;61(4):841–858. doi: 10.1093/genetics/61.4.841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ruddle F. H., Shows T. B., Roderick T. H. Autosomal control of an electrophoretic variant of glucose-6-phosphate dehydrogenase in the mouse (Mus musculus). Genetics. 1968 Apr;58(4):599–606. doi: 10.1093/genetics/58.4.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Selander R. K., Yang S. Y. Protein polymorphism and genic heterozygosity in a wild population of the house mouse (Mus musculus). Genetics. 1969 Nov;63(3):653–667. doi: 10.1093/genetics/63.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shaw C. R., Prasad R. Starch gel electrophoresis of enzymes--a compilation of recipes. Biochem Genet. 1970 Apr;4(2):297–320. doi: 10.1007/BF00485780. [DOI] [PubMed] [Google Scholar]
  16. Steele M. W., Young W. J., Childs B. Glucose 6-phosphate dehydrogenase in Drosophila malanogaster: starch gel electrophoretic variation due to molecular instability. Biochem Genet. 1968 Sep;2(2):159–175. doi: 10.1007/BF01458714. [DOI] [PubMed] [Google Scholar]
  17. Stone W. S., Wheeler M. R., Johnson F. M., Kojima K. I. Genetic variation in natural island populations of members of the Drosophila nasuta and Drosophila ananassae subgroups. Proc Natl Acad Sci U S A. 1968 Jan;59(1):102–109. doi: 10.1073/pnas.59.1.102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sullivan B., Nute P. E. Structural and functional properties of polymorphic hemoglobins from orangutans. Genetics. 1968 Jan;58(1):113–124. doi: 10.1093/genetics/58.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tait A. Genetics of NADP isocitrate dehydrogenase in Paramecium aurelia. Nature. 1970 Jan 10;225(5228):181–182. doi: 10.1038/225181a0. [DOI] [PubMed] [Google Scholar]
  20. Ursprung H., Carlin L. Drosophila alcohol dehydrogenase: in vitro changes of isozyme patterns. Ann N Y Acad Sci. 1968 Jun 14;151(1):456–475. doi: 10.1111/j.1749-6632.1968.tb11908.x. [DOI] [PubMed] [Google Scholar]
  21. VAUGHAN M., STEINBERG D. The specificity of protein biosynthesis. Adv Protein Chem. 1959;14:115–173. doi: 10.1016/s0065-3233(08)60610-5. [DOI] [PubMed] [Google Scholar]
  22. Whitt G. S. Homology of lactate dehydrogenase genes: E gene function in the teleost nervous system. Science. 1969 Nov 28;166(3909):1156–1158. doi: 10.1126/science.166.3909.1156. [DOI] [PubMed] [Google Scholar]

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