Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 May;68(5):1010–1013. doi: 10.1073/pnas.68.5.1010

A New Photosynthetic Pigment, “P430”: Its Possible Role as the Primary Electron Acceptor of Photosystem I

Tetsuo Hiyama 1, Bacon Ke 1
PMCID: PMC389102  PMID: 4995817

Abstract

The technique of flash kinetic spectrophotometry was used to demonstrate a broad absorption band around 430 nm, which was kinetically different from P700, in several photosystem-I particles from spinach and blue-green algae. The component represented by this absorption band, designated as “P430”, was bleached as fast as P700. Its recovery in the dark was accelerated by ferredoxin and by various artificial electron acceptors with redox potentials as low as -521 mV. The recovery kinetics have been demonstrated to be identical to those of a concomitant reduction of several of the artificial electron acceptors used. Tentatively, “P430” has been proposed as a possible primary electron acceptor of photosystem I.

Full text

PDF
1010

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. M., Boardman N. K. Fractionation of the photochemical systems of photosynthesis. I. Chlorophyll contents and photochemical activities of particles isolated from spinach chloroplasts. Bibl Laeger. 1966 Mar 14;112(3):403–421. doi: 10.1016/0926-6585(66)90244-5. [DOI] [PubMed] [Google Scholar]
  2. Arnon D. I. Ferredoxin and photosynthesis. Science. 1965 Sep 24;149(3691):1460–1470. doi: 10.1126/science.149.3691.1460. [DOI] [PubMed] [Google Scholar]
  3. Black C. C., Jr Chloroplast reactions with dipyridyl salts. Biochim Biophys Acta. 1966 Jul 13;120(3):332–340. doi: 10.1016/0926-6585(66)90300-1. [DOI] [PubMed] [Google Scholar]
  4. Clayton R. K., Straley S. C. An optical absorption change that could be due to reduction of the primary photochemical electron acceptor in photosynthetic reaction centers. Biochem Biophys Res Commun. 1970;39(6):1114–1119. doi: 10.1016/0006-291x(70)90674-1. [DOI] [PubMed] [Google Scholar]
  5. Fuller R. C., Nugent N. A. Pteridines and the function of the photosynthetic reaction center. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1311–1318. doi: 10.1073/pnas.63.4.1311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hiyama T., Ke B. Laser-induced reactions of P700 and cytochrome f in a blue-green alga, Plectonema boryanum. Biochim Biophys Acta. 1971 Mar 2;226(2):320–327. doi: 10.1016/0005-2728(71)90099-5. [DOI] [PubMed] [Google Scholar]
  7. KE B. LIGHT-INDUCED RAPID ABSORPTION CHANGES DURING PHOTOSYNTHESIS. V. DIGITONIN-TREATED CHLOROPLASTS. Biochim Biophys Acta. 1964 Sep 25;88:297–303. doi: 10.1016/0926-6577(64)90185-8. [DOI] [PubMed] [Google Scholar]
  8. Kassner R. J., Kamen M. D. The photoreduction of spinach ferredoxin in the presence of porphyrin and an electron donor. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2445–2450. doi: 10.1073/pnas.58.6.2445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ke B., Ogawa T., Hiyama T., Vernon L. P. Experimental determination of the molar differential extinction coefficient of P700. Biochim Biophys Acta. 1971 Jan 12;226(1):53–62. doi: 10.1016/0005-2728(71)90177-0. [DOI] [PubMed] [Google Scholar]
  10. Kok B., Rurainski H. J., Owens O. V. The reducing power generated in photoact I of photosynthesis. Biochim Biophys Acta. 1965 Nov 29;109(2):347–356. doi: 10.1016/0926-6585(65)90162-7. [DOI] [PubMed] [Google Scholar]
  11. Malkin R., Bearden A. J. Primary reactions of photosynthesis: photoreduction of a bound chloroplast ferredoxin at low temperature as detected by EPR spectroscopy. Proc Natl Acad Sci U S A. 1971 Jan;68(1):16–19. doi: 10.1073/pnas.68.1.16. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ogawa T., Vernon L. P. A fraction from Anabaena variabilis enriched in the reaction center chlorophyll P700. Biochim Biophys Acta. 1969 Jun 24;180(2):334–346. doi: 10.1016/0005-2728(69)90118-2. [DOI] [PubMed] [Google Scholar]
  13. Ogawa T., Vernon L. P., Mollenhauer H. H. Properties and structure of fractions prepared from Anabaena variabilis by the action Triton X-100. Biochim Biophys Acta. 1969 Feb 25;172(2):216–229. doi: 10.1016/0005-2728(69)90065-6. [DOI] [PubMed] [Google Scholar]
  14. Ogawa T., Vernon L. P. Properties of partially purified photosynthetic reaction centers from Scenedesmus mutant 6E and Anabaena variabilis grown in the presence of diphenylamine. Biochim Biophys Acta. 1970 Mar 3;197(2):292–301. doi: 10.1016/0005-2728(70)90040-x. [DOI] [PubMed] [Google Scholar]
  15. RUMBERG B., WITT H. T. ANALYSE DER PHOTOSYNTHESE MIT BLITZLICHT. I. DIE PHOTOOXYDATION VON CHLOROPHYLL-A1-430-703. Z Naturforsch B. 1964 Aug;19:693–707. [PubMed] [Google Scholar]
  16. Wang J. H. Oxidative and photosynthetic phosphorylation mechanisms. Science. 1970 Jan 2;167(3914):25–30. doi: 10.1126/science.167.3914.25. [DOI] [PubMed] [Google Scholar]
  17. Yocum C. F., San Pietro A. Ferredoxin reducing substance (FRS) from spinach. Biochem Biophys Res Commun. 1969 Aug 15;36(4):614–620. doi: 10.1016/0006-291x(69)90349-0. [DOI] [PubMed] [Google Scholar]
  18. Zweig G., Shavit N., Avron M. Diquat (I,I'-ethylene-2,2'-dipyridylium dibromide) in photo-reactions of isolated chloroplasts. Biochim Biophys Acta. 1965 Nov 29;109(2):332–346. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES