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. 1971 Jun;68(6):1157–1161. doi: 10.1073/pnas.68.6.1157

Heterogeneity, Complexity, and Repetition of the Chloroplast DNA of Chlamydomonas reinhardtii

Deepak Bastia 1,2, Kwen-Sheng Chiang 1,2, Hewson Swift 1,2, Peter Siersma 1,2
PMCID: PMC389141  PMID: 4942181

Abstract

The chloroplast DNA of wild-type Chlamydomonas reinhardtii was isolated in a CsCl density gradient as a single, homogeneous density class with a mean density of 1.695 g/cm3. Irrespective of sheared size, denatured chloroplast DNA renatured as a single homogeneous species. Compositional heterogeneity, presumably intramolecular, was revealed by the absorbance-temperature profile. The complement of unique nucleotide sequences of the chloroplast DNA, as determined by the rate of renaturation, was 1.94 × 108 daltons. This kinetic complexity is 26-fold less than the DNA content of a single gamete chloroplast, and 52-fold less than the chloroplast of a vegetative cell, which indicates that the chloroplast of C. reinhardtii possesses at least 26 copies of a unique nucleotide sequence.

Keywords: Tm, density gradient centrifugation, reannealing, hydroxyapatite chromatography

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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