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. 1971 Jun;68(6):1184–1187. doi: 10.1073/pnas.68.6.1184

Extrinsic Cotton Effects in Hapten—Carrier and Hapten—Antibody Interactions

Vincent Reid 1, Michael Glaser 1, Roger Kennett 1, S J Singer 1,*
PMCID: PMC389148  PMID: 4108175

Abstract

Two homogeneous univalent hapten-protein conjugates, prepared by the covalent attachment of a single 2,4-dinitrophenyl (DNP-) or 2,4,6-trinitrophenyl (TNP-) side chain to the cysteine-SH in the active site of the enzyme papain, have been found to exhibit large Cotton effects in the wavelength region of the absorption bands of the DNP or TNP groups. This indicates that the DNP or TNP groups are noncovalently bound to some asymmetric region(s) of the papain molecule. These homogeneous papain conjugates are antigens that can elicit anti-DNP or anti-TNP antibody production in mice or rabbits. It is suggested that the noncovalent binding of a hapten to the surface of its carrier protein may profoundly affect the characteristics of the anti-hapten antibodies that are elicited. It has also been observed that the specific binding of these papain conjugates, and of the simple haptens DNP-lysine or TNP-lysine, to anti-hapten antibodies produces characteristic extrinsic Cotton effects.

Keywords: hapten binding, DNP-papain, antigenic determinants

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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