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. 1971 Jun;68(6):1286–1288. doi: 10.1073/pnas.68.6.1286

Mechanism of Action of 1,25-Dihydroxycholecalciferol on Intestinal Calcium Transport

Y Tanaka 1, H F DeLuca 1,*, J Omdahl 1, M F Holick 1
PMCID: PMC389173  PMID: 4331086

Abstract

The prior administration of actinomycin D prevents the metabolism of [3H]25-hydroxycholecalciferol to 1,25-dihydroxycholecalciferol, a metabolite of vitamin D3 that is effective in the stimulation of intestinal calcium transport. In this paper, the question of whether the response of intestinal calcium transport to 1,25-dihydroxycholecalciferol is sensitive to actinomycin D was examined. While the response of intestinal transport to physiological amounts of 25-hydroxycholecalciferol is blocked by actinomycin D, the response of intestinal calcium transport to 1,25-dihydroxycholecalciferol is insensitive to the antibiotic. These results suggest that 1,25-dihydroxycholecalciferol, or a further metabolite thereof, is the metabolically active form of vitamin D in the intestine, that it functions by a process not involving transcription of DNA, and that the step sensitive to actinomycin D in the action of vitamin D on the intestine does not occur in the intestine, but is the conversion of 25-hydroxycholecalciferol to 1,25-dihydroxycholecalciferol in the kidney.

Keywords: rat, vitamin D, actinomycin D, kidney, “peak V” metabolite

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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