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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Jun;68(6):1312–1315. doi: 10.1073/pnas.68.6.1312

A Bovine Pancreatic Enzyme Catalyzing the Conversion of Proinsulin to Insulin

C C Yip 1
PMCID: PMC389179  PMID: 5288380

Abstract

An enzyme that catalyzes the conversion of bovine proinsulin to insulin has been purified from a bovine pancreatic extract. The product of conversion was identified as insulin by aminoacid analysis and determination of carboxyl terminal aminoacid residues. The purified enzyme preparation showed one major protein band on polyacrylamide gel disc electrophoresis; it had a molecular weight of about 70,000 and an isoelectric point (pI) at a pH of 4.82.

Keywords: amino acid analysis, gel electrophoresis, tryptic activity, molecular weight

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Selected References

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