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. 1971 Jun;68(6):1346–1350. doi: 10.1073/pnas.68.6.1346

In Vitro Detection of Cytotoxic Cellular Immunity Against Tumor-Specific Antigens by a Radioisotopic Technique

S M Jagarlamoody 1,2, J C Aust 1,2, R H Tew 1,2, C F McKhann 1,2
PMCID: PMC389186  PMID: 4331089

Abstract

[3H]Thymidine-labeled tumor cells are used to evaluate the cytotoxic cellular immune response against tumor-specific antigens; the loss of label due to destruction and detachment of target cells from the surface of the culture vessel is measured. Spleen cells from mice immunized against Moloney virus-induced rhabdomyosarcoma specifically destroyed the sarcoma cells, while cells from normal syngeneic mice did not. Peripheral blood lymphocytes from patients with malignant tumors were specifically cytotoxic to autologous tumor cells and to allogeneic tumor cells histopathologically identical to the autologous tumor, but not to autologous nonmalignant fibroblasts, or to allogeneic tumor cells from a histologically dissimilar tumor. Serum from the same patients specifically protected autologous tumor cells from lymphocyte cytotoxicity. This serum-mediated protection of tumor cells against autologous cellular immunocytotoxicity also extended to histologically identical allogeneic tumor cells. Cross-reactivity of anti-tumor cellular immunocytotoxicity in vitro, and its “blocking” by autologous serum, strongly suggest the presence of common tumor antigens. The antagonism demonstrated in vitro between serum and cellular immunity may explain the continued growth of malignant tumors in the face of demonstrable cellular immunity.

Keywords: virus, methylcholanthrene, human tumors, mouse, tumor-specific antigens, blocking antibody

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brunner K. T., Mauel J., Cerottini J. C., Chapuis B. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 1968 Feb;14(2):181–196. [PMC free article] [PubMed] [Google Scholar]
  2. Brunner K. T., Mauel J., Schindler R. In vitro studies of cell-bound immunity; cloning assay of the cytotoxic action of sensitized lymphoid cells on allogeneic target cells. Immunology. 1966 Nov;11(5):499–506. [PMC free article] [PubMed] [Google Scholar]
  3. Bubeník J., Perlmann P., Helmstein K., Moberger G. Immune response to urinary bladder tumours in man. Int J Cancer. 1970 Jan 15;5(1):39–46. doi: 10.1002/ijc.2910050106. [DOI] [PubMed] [Google Scholar]
  4. Fefer A., McCoy J. L., Glynn J. P. Induction and regression of primary moloney sarcoma virus-induced tumors in mice. Cancer Res. 1967 Sep;27(9):1626–1631. [PubMed] [Google Scholar]
  5. Fefer A., McCoy J. L., Perk K., Glynn J. P. Immunologic, virologic, and pathologic studies of regression of autochthonous Moloney sarcoma virus-induced tumors in mice. Cancer Res. 1968 Aug;28(8):1577–1585. [PubMed] [Google Scholar]
  6. GOVAERTS A. Cellular antibodies in kidney homotransplantation. J Immunol. 1960 Nov;85:516–522. [PubMed] [Google Scholar]
  7. Hellström I. E., Hellström K. E., Pierce G. E., Bill A. H. Demonstration of cell-bound and humoral immunity against neuroblastoma cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1231–1238. doi: 10.1073/pnas.60.4.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hellström I. A colony inhibition (CI) technique for demonstration of tumor cell destruction by lymphoid cells in vitro. Int J Cancer. 1967 Jan 15;2(1):65–68. doi: 10.1002/ijc.2910020111. [DOI] [PubMed] [Google Scholar]
  9. Hellström I., Evans C. A., Hellström K. E. Cellular immunity and its serum-mediated inhibition in Shope-virus-induced rabbit papillomas. Int J Cancer. 1969 Sep 15;4(5):601–607. doi: 10.1002/ijc.2910040504. [DOI] [PubMed] [Google Scholar]
  10. Hellström I., Hellström K. E. Studies on cellular immunity and its serum mediated inhibition in Moloney-virus-induced mouse sarcomas. Int J Cancer. 1969 Sep 15;4(5):587–600. doi: 10.1002/ijc.2910040503. [DOI] [PubMed] [Google Scholar]
  11. Ming S. C., Klein E., Klein G. Inhibitory effect of lymphocytes on DNA synthesis of target cells in vitro. Ann Med Exp Biol Fenn. 1966;44(2):191–200. [PubMed] [Google Scholar]
  12. PERLMANN P., BROBERGER O. In vitro studies of ulcerative colitis. II. Cytotoxic action of white blood cells from patients on human fetal colon cells. J Exp Med. 1963 May 1;117:717–733. doi: 10.1084/jem.117.5.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pearson G. R., Hodes R. J., Friberg S. Cytotoxic potential of different lymphoid cell populations against chromium-51 labelled tumour cells. Clin Exp Immunol. 1969 Sep;5(3):273–284. [PMC free article] [PubMed] [Google Scholar]
  14. ROSENAU W., MOON H. D. THE SPECIFICITY OF THE CYTOLYTIC EFFECT OF SENSITIZED LYMPHOID CELLS IN VITRO. J Immunol. 1964 Dec;93:910–914. [PubMed] [Google Scholar]
  15. Takasugi M., Klein E. A microassay for cell-mediated immunity. Transplantation. 1970 Mar;9(3):219–227. doi: 10.1097/00007890-197003000-00005. [DOI] [PubMed] [Google Scholar]

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