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. 1971 Jul;68(7):1425–1427. doi: 10.1073/pnas.68.7.1425

Expression of Two X-Linked Genes in Human Hair Follicles of Double Heterozygotes

Joseph L Goldstein *, James F Marks †, Stanley M Gartler *
PMCID: PMC389209  PMID: 5283930

Abstract

Expression of the two X-linked loci glucose-6-phosphate dehydrogenase (G6PD; EC 1.1.1.49) and hypoxanthine:guanine phosphoribosyltransferase (HGPRT; EC 2.4.2.8) was studied in single hair follicles of two females who were heterozygous for both of these genes (double heterozygotes). The coupling phase for these two loci was known to be g6pd A and hyprt- on the maternal X chromosome and g6pd B and hgprt+ on the paternal X. Three phenotypic classes of hair follicles were observed in both double heterozygotes: G6pd A follicles with deficient HGPRT activity, G6pd B follicles with normal HGPRT activity, and G6pd AB follicles with intermediate HGPRT activity. These data directly demonstrate one of the predictions of the Lyon hypothesis that for two X-linked loci, those genes in cis position are turned on or off in a cell and its clone, while in trans only one gene or the other is expressed.

Keywords: Lesch-Nyhan syndrome, gene inactivation, Lyon hypothesis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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