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. 1971 Jul;68(7):1469–1473. doi: 10.1073/pnas.68.7.1469

Bacterial Mutants Defective in Plasmid Formation: Requirement for the lon+ Allele

Toshiya Takano 1,*
PMCID: PMC389220  PMID: 4934518

Abstract

Bacterial mutants defective in plasmid formation were selected for their inability to be transduced to chloramphenicol resistance by bacteriophage PlCM. The mutants isolated are indistinguishable from a lon mutant strain. Both the lon strain and the mutants isolated here show very poor lysogenization by P1 and very low transduction to Gal+ by the plasmid formation of the λgal8-Nam7am53cI857. The lon+ gene function of the host bacteria is indispensable for plasmid formation, even though P1 and λ+ can grow normally on the lon strains and λ+ can be integrated normally in lon as well as in the lon+ bacteria. Phage mutants that can persist as plasmids in lon strains were isolated from P1CM. The function of the lon+ gene is discussed with regard to plasmid formation.

Keywords: transduction, phage P1, E. coli, lysogeny, UV reduce sensitivity

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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