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. 1971 Jul;68(7):1525–1529. doi: 10.1073/pnas.68.7.1525

Components of Adenovirus-Simian Virus 40 Hybrid Viruses

Stephen G Baum 1,2, Robert I Fox 1,2
PMCID: PMC389232  PMID: 4327006

Abstract

The DNA and protein components of the adenovirus 7-simian virus (SV)40 hybrid virus “E46+” have been investigated. The Ad.7-SV40 hybrid DNA genome could be distinguished from nonhybrid Ad. 7 genome on the basis of its molecular weight in an alkaline sucrose gradient. The hybrid genome could also be separated from nonhybrid adenovirus 2 DNA when purified transcapsidant adenovirus 2+t7 (Ad.7-SV40 DNA within an Ad.2 capsid) was studied. The presence and amount of SV40 DNA in the hybrid virus genome was determined by DNA-RNA hybridization. The results presented suggest: (i) that about 10% of the particles in the E46+ preparations contained SV40 DNA covalently linked to adenovirus DNA; (ii) that the hybrid virus genome has a molecular weight 10-12% lower than that of nonhybrid virus, consistent with a deletion of adenovirus DNA; and (iii) that the hybrid virus contained only 40-50% of a total SV40 genome.

The protein components of the Ad.7-SV40 hybrid virus were examined by acrylamide gel electrophoresis in sodium dodecyl sulfate. No significant quantitative or qualitative differences between the proteins of hybrid and nonhybrid adenovirus were demonstrated.

Keywords: hybridization, density gradient centrifugation, gel electrophoresis, DNA, proteins

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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