Abstract
Studies of the amino acid sequences around half-cystine residues in an immunoglobulin M have revealed an unexpectedly high number. At least 14 different sequences were found in the μ chain (VHIII subclass). Four of these were involved in interchain disulfide bridges and at least 10 in intrachain bridges. Five came from the κ chain (κIII subclass, Inv b). Several others, although having a high degree of homology, were not identical with those of either the μ or κ chains. These results support the concept of an additional fragment in γM molecules, although its function and localization remain to be determined.
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Selected References
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- Beale D., Buttress N. Studies on a human 19-S immunoglobulin M. The arrangement of inter-chain disulphide bridges and carbohydrate sites. Biochim Biophys Acta. 1969 May;181(1):250–267. doi: 10.1016/0005-2795(69)90248-7. [DOI] [PubMed] [Google Scholar]
- Beale D., Feinstein A. Evidence for C-terminal intra-subunit disulphide bridges between immunoglobulin-M heavy chains. FEBS Lett. 1970 Apr 2;7(2):175–176. doi: 10.1016/0014-5793(70)80149-1. [DOI] [PubMed] [Google Scholar]
- Beale D., Feinstein A. Studies on the reduction of a human 19S immunoglobulin M. Biochem J. 1969 Apr;112(2):187–194. doi: 10.1042/bj1120187. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frangione B., Milstein C., Pink J. R. Structural studies of immunoglobulin G. Nature. 1969 Jan 11;221(5176):145–148. doi: 10.1038/221145a0. [DOI] [PubMed] [Google Scholar]
- Frangione B., Milstein C. Variations in the S-S bridges of immunoglobins G: interchain disulfide bridges of gamma G3 myeloma proteins. J Mol Biol. 1968 May 14;33(3):893–906. doi: 10.1016/0022-2836(68)90326-4. [DOI] [PubMed] [Google Scholar]
- Franklin E. C., Frangione B. Structural differences between macroglobulins belonging to two serologically distinguishable subclasses. Biochemistry. 1968 Dec;7(12):4203–4211. doi: 10.1021/bi00852a010. [DOI] [PubMed] [Google Scholar]
- Halpern M. S., Koshland M. E. Noval subunit in secretory IgA. Nature. 1970 Dec 26;228(5278):1276–1278. doi: 10.1038/2281276a0. [DOI] [PubMed] [Google Scholar]
- Kohler H., Shimizu A., Paul C., Putnam F. W. Macroglobulin structure: variable sequence of light and heavy chains. Science. 1970 Jul 3;169(3940):56–59. doi: 10.1126/science.169.3940.56. [DOI] [PubMed] [Google Scholar]
- Mestecky J., Zikan J., Butler W. T. Immunoglobulin M and secretory immunoglobulin A: presence of a common polypeptide chain different from light chains. Science. 1971 Mar 19;171(3976):1163–1165. doi: 10.1126/science.171.3976.1163. [DOI] [PubMed] [Google Scholar]
- Metzger H. Structure and function of gamma M macroglobulins. Adv Immunol. 1970;12:57–116. doi: 10.1016/s0065-2776(08)60168-6. [DOI] [PubMed] [Google Scholar]
- Miekka S. I., Deutsch H. F. The primary structure of a tetradecapeptide containing the cysteine residue linking gamma M-globulin subunits. J Biol Chem. 1970 Nov 10;245(21):5534–5544. [PubMed] [Google Scholar]
- Miller F., Metzger H. Characterization of a human macroglobulin. II. Distribution of the disulfide bonds. J Biol Chem. 1965 Dec;240(12):4740–4745. [PubMed] [Google Scholar]
- Milstein C., Pink J. R. Structure and evolution of immunoglobulins. Prog Biophys Mol Biol. 1970;21:209–263. doi: 10.1016/0079-6107(70)90026-x. [DOI] [PubMed] [Google Scholar]
- Paul C., Shimizu A., Köhler H., Putnam F. W. Structure of the hinge region of the mu heavy chain of human IgM immunoglobulins. Science. 1971 Apr 2;172(3978):69–72. doi: 10.1126/science.172.3978.69. [DOI] [PubMed] [Google Scholar]
- Pink J. R., Milstein C. Inter heavy-light chain disulphide bridge in immune globulins. Nature. 1967 Apr 1;214(5083):92–94. doi: 10.1038/214092a0. [DOI] [PubMed] [Google Scholar]
- Plaut A. G., Tomasi T. B., Jr Immunoglobulin M: pentameric Fcmu fragments released by trypsin at higher temperatures. Proc Natl Acad Sci U S A. 1970 Feb;65(2):318–322. doi: 10.1073/pnas.65.2.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suzuki T., Deutsch H. F. Dissociation, reaggregation, and subunit structure studies of some human gamma-M-globulins. J Biol Chem. 1967 Jun 10;242(11):2725–2738. [PubMed] [Google Scholar]
- Terry W. D., Ohms J. Implications of heavy chain disease protein sequences for multiple gene theories of immunoglobulin synthesis. Proc Natl Acad Sci U S A. 1970 Jun;66(2):558–563. doi: 10.1073/pnas.66.2.558. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wikler M., Köhler H., Shinoda T., Putnam F. W. Macroglobulin structure: homology of mu and gamma heavy chains of human immunoglobulins. Science. 1969 Jan 3;163(3862):75–78. doi: 10.1126/science.163.3862.75. [DOI] [PubMed] [Google Scholar]