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. 1971 Jul;68(7):1590–1593. doi: 10.1073/pnas.68.7.1590

Prolonged Survival of Glutaraldehyde-Treated Skin Homografts

Israel Schechter 1,*
PMCID: PMC389247  PMID: 4934523

Abstract

Treatment of mouse skin homografts in vitro with glutaraldehyde prolonged their average survival time from 12.4 to 39.2 days, presumably because the reagent became covalently bound to the histocompatibility antigen sites (or in their close vicinity) and shielded them from the immune apparatus of the recipient. The attachment to skin of the inert polymer poly(L-lysine) via this bifunctional reagent increased the average survival time to 52.9 days. The simplicity and versatility of this approach might make it possible to screen a large number of reagents that can bind covalently to tissue constituents under physiological conditions. It seems possible that a particular treatment leading to a new contact surface in the transplant might favor the acceptance of homografts as well as of heterografts.

Keywords: mice, poly(L-lysine), polypeptides

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbott W. M., Pappas A. M. Comparative studies on fresh and preserved skin: fundamental biologic differences in behavior as grafts. Ann Surg. 1970 Nov;172(5):781–786. doi: 10.1097/00000658-197011000-00002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BILLINGHAM R. E., KROHN P. L., MEDAWAR P. B. Effect of locally applied cortisone acetate on survival of skin homografts in rabbits. Br Med J. 1951 Nov 3;2(4739):1049–1053. doi: 10.1136/bmj.2.4739.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bauminger S., Schechter I., Sela M. Specificity of immunological tolerance to poly-DL-aalanyl proteins. Immunochemistry. 1967 May;4(3):169–181. doi: 10.1016/0019-2791(67)90126-7. [DOI] [PubMed] [Google Scholar]
  4. Bonmassar E., Francesconi G., Manzoni S. C., Perelli-Ercolini M. Chemical deletion of histocompatibility antigens--homograft survival of rat skin treated with "Urethan" in vitro. Nature. 1966 Mar 12;209(5028):1141–1142. doi: 10.1038/2091141a0. [DOI] [PubMed] [Google Scholar]
  5. DUKES C. D., BLOCKER T. G., Jr Studies on the survival of skin homografts. I. Prolongation of life of full-thickness grafts by the action of streptokinase-streptodornase. Ann Surg. 1952 Dec;136(6):999–1006. doi: 10.1097/00000658-195212000-00013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GRILLO H. C., MCKHANN C. F. THE ACCEPTANCE AND EVOLUTION OF DERMAL HOMOGRAFTS FREED OF VIABLE CELLS. Transplantation. 1964 Jan;2:48–59. doi: 10.1097/00007890-196401000-00006. [DOI] [PubMed] [Google Scholar]
  7. HELLMANN K., DUKE D. I., TUCKER D. F. PROLONGATION OF SKIN HOMOGRAFT SURVIVAL BY THALIDOMIDE. Br Med J. 1965 Sep 18;2(5463):687–689. doi: 10.1136/bmj.2.5463.687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lemperle G. Prolonged survival of skin allografts after incubation with recipient DNA or RNA. J Surg Res. 1968 Nov;8(11):511–521. doi: 10.1016/0022-4804(68)90001-2. [DOI] [PubMed] [Google Scholar]
  9. PARRISH R. A., TIPPENS J. K., PULLIAM M. M., MORETZ W. H. FORMALIZED SKIN SUBSTITUTE FOR GRAFTING BURNS: A PRELIMINARY REPORT. Am Surg. 1964 Nov;30:793–798. [PubMed] [Google Scholar]
  10. Pruitt B. A., Jr, Moncrief J. A. Current trends in burn research. II. J Surg Res. 1967 Jul;7(7):332–347. doi: 10.1016/0022-4804(67)90075-3. [DOI] [PubMed] [Google Scholar]
  11. Staats J. Standardized nomenclature for inbred strains of mice: fourth listing. Cancer Res. 1968 Mar;28(3):391–420. [PubMed] [Google Scholar]
  12. White E., Hildemann W. H. Allografts in genetically defined rats: difference in survival between kidney and skin. Science. 1968 Dec 13;162(3859):1293–1295. doi: 10.1126/science.162.3859.1293. [DOI] [PubMed] [Google Scholar]
  13. Williams G. M., DePlanque B., Graham W. H., Lower R. R. Participation of antibodies in acute cardiac-allograft rejection in man. N Engl J Med. 1969 Nov 20;281(21):1145–1150. doi: 10.1056/NEJM196911202812103. [DOI] [PubMed] [Google Scholar]
  14. Williams G. M., Hume D. M., Hudson R. P., Jr, Morris P. J., Kano K., Milgrom F. "Hyperacute" renal-homograft rejection in man. N Engl J Med. 1968 Sep 19;279(12):611–618. doi: 10.1056/NEJM196809192791201. [DOI] [PubMed] [Google Scholar]
  15. Zaroff L. I., Mills W., Jr, Duckett J. W., Jr, Switzer W. E., Moncrief J. A. Multiple uses of viable cutaneous homografts in the burned patient. Surgery. 1966 Mar;59(3):368–372. [PubMed] [Google Scholar]

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