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. 1971 Jul;68(7):1644–1647. doi: 10.1073/pnas.68.7.1644

Immunological Tolerance: “Forbidden Clones” Allowed in Tetraparental Mice

T G Wegmann 1,2,3, I Hellström 1,2,3, K E Hellström 1,2,3
PMCID: PMC389259  PMID: 5283958

Abstract

Tetraparental (allophenic) mice, made chimeric at the eight-cell stage by joining two embryos from histoincompatible parental strains, were examined by a microcytotoxicity test. The results indicate that parental-strain fibroblasts are more effectively destroyed in vitro by lymph node cells from the tetraparental mice than by lymph node cells from the F1 hybrid or either parental stain. The destruction by tetraparental lymph node cells is indistinguishable from that mediated by lymph node cells from previously immunized allogeneic animals. It can be prevented by serum from the tetraparental mice, but not by sera from the F1 hybrid or the parental strain animals. The results suggest that a concomitant immunity and serum blocking effect, rather than a central failure of the immune response, may mediate some aspects of normal tolerance.

Keywords: serum blocking factor

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAILEY D. W. Mosaic histocompatibility of skin grafts from female mice. Science. 1963 Aug 16;141(3581):631–633. doi: 10.1126/science.141.3581.631. [DOI] [PubMed] [Google Scholar]
  2. BILLINGHAM R. E., BRENT L., MEDAWAR P. B. Actively acquired tolerance of foreign cells. Nature. 1953 Oct 3;172(4379):603–606. doi: 10.1038/172603a0. [DOI] [PubMed] [Google Scholar]
  3. Boyse E. A., Lance E. M., Carswell E. A., Cooper S., Old L. J. Rejection of skin allografts by radiation chimaeras: selective gene action in the specification of cell surface structure. Nature. 1970 Aug 29;227(5261):901–903. doi: 10.1038/227901a0. [DOI] [PubMed] [Google Scholar]
  4. Hellström I., Hellström K. E., Allison A. C. Neonatally induced allograft tolerance may be mediated by serum-borne factors. Nature. 1971 Mar 5;230(5288):49–50. doi: 10.1038/230049a0. [DOI] [PubMed] [Google Scholar]
  5. Hellström K. E., Hellström I. Immunological enhancement as studied by cell culture techniques. Annu Rev Microbiol. 1970;24:373–398. doi: 10.1146/annurev.mi.24.100170.002105. [DOI] [PubMed] [Google Scholar]
  6. Mintz B., Silvers W. K. "Intrinsic" immunological tolerance in allophenic mice. Science. 1967 Dec 15;158(3807):1484–1486. doi: 10.1126/science.158.3807.1484. [DOI] [PubMed] [Google Scholar]
  7. TARKOWSKI A. K. Mouse chimaeras developed from fused eggs. Nature. 1961 Jun 3;190:857–860. doi: 10.1038/190857a0. [DOI] [PubMed] [Google Scholar]
  8. Voisin G. A., Kinsky R. G., Maillard J. Réativité immunitaire et anticorps facilitants chez des animaux tolérants aux homogreffes. Ann Inst Pasteur (Paris) 1968 Nov;115(5):855–879. [PubMed] [Google Scholar]
  9. Warner N. L., Herzenberg L. A., Cole L. J., Davis W. E., Jr Dissociation of skin homograft tolerance and donor type gamma globulin synthesis in allogeneic mouse radiation chimaeras. Nature. 1965 Mar 13;205(976):1077–1079. doi: 10.1038/2051077a0. [DOI] [PubMed] [Google Scholar]
  10. Wegman T. G. Enzyme patterns in tetraparental mouse liver. Nature. 1970 Jan 31;225(5231):462–463. doi: 10.1038/225462a0. [DOI] [PubMed] [Google Scholar]
  11. Wegmann T. G., Gilman J. G. Chimerism for three genetic systems in tetraparental mice. Dev Biol. 1970 Mar;21(3):281–291. doi: 10.1016/0012-1606(70)90125-9. [DOI] [PubMed] [Google Scholar]
  12. Wilson D. B., Billingham R. E. Lymphocytes and transplantation immunity. Adv Immunol. 1967;7:189–273. doi: 10.1016/s0065-2776(08)60129-7. [DOI] [PubMed] [Google Scholar]

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