Abstract
The proliferation of chicken fibroblasts is strongly stimulated by folic acid, at greater than physiological concentrations, in a plasma-containing medium, or by serum in a medium of physiological folic acid concentration. Serum and plasma, however, do not appear to differ in their content of folic acid derivatives.
A qualitative difference between the proliferative behaviors of normal and transformed fibroblasts is obtained when these cells are cultivated in a physiological-folate-low-calcium-plasma medium. In such a medium, transformants proliferate steadily, at a significant rate, while normal fibroblasts do not multiply at all.
Keywords: calcium, transformed fibroblasts
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Selected References
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- BECKER C., BEAUDREAU G. S., CASTLE W., GIBSON B. W., BEARD D., BEARD J. W. Virus of avian myeloblastosis. XXII. Influence of folic acid and other factors on growth of myeloblasts and virus synthesis in tissue culture. J Natl Cancer Inst. 1962 Sep;29:455–481. [PubMed] [Google Scholar]
- Balk S. D. Calcium as a regulator of the proliferation of normal, but not of transformed, chicken fibroblasts in a plasma-containing medium. Proc Natl Acad Sci U S A. 1971 Feb;68(2):271–275. doi: 10.1073/pnas.68.2.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EAGLE H. Nutrition needs of mammalian cells in tissue culture. Science. 1955 Sep 16;122(3168):501–514. doi: 10.1126/science.122.3168.501. [DOI] [PubMed] [Google Scholar]
- FISCHER G. A. Studies of the culture of leukemic cells in vitro. Ann N Y Acad Sci. 1958 Dec 5;76(3):673–680. doi: 10.1111/j.1749-6632.1958.tb54884.x. [DOI] [PubMed] [Google Scholar]
- HAFF R. F., SWIM H. E. Minimal vitamin requirements of rabbit fibroblasts, strain RM3-73. Proc Soc Exp Biol Med. 1957 Apr;94(4):779–782. doi: 10.3181/00379727-94-23085. [DOI] [PubMed] [Google Scholar]
- LIEBERMAN I., OVE P. Control of growth of mammalian cells in culture with folic acid, thymidine, and purines. J Biol Chem. 1960 Apr;235:1119–1123. [PubMed] [Google Scholar]
- NEUMAN R. E., TYTELL A. A. Stimulatory effects of glycine, L-serine, folic acid and related compounds on growth of cell cultures. Proc Soc Exp Biol Med. 1960 Apr;103:763–767. doi: 10.3181/00379727-103-25663. [DOI] [PubMed] [Google Scholar]
- SCHEINDLIN S., GRIFFITH I. The action of ascorbic acid on folic acid. Am J Pharm Sci Support Public Health. 1951 Mar;123(3):78–86. [PubMed] [Google Scholar]