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. 1971 Aug;68(8):1828–1832. doi: 10.1073/pnas.68.8.1828

Mechanism of Initiation of Repression of In Vitro Transcription of the Lac Operon of Escherichia coli

Larry Eron *, Ricardo Block †
PMCID: PMC389302  PMID: 4331560

Abstract

A cyclic AMP-binding protein (CAP protein), cyclic AMP, and RNA polymerase holoenzyme are shown to initiate lac transcription at the lac promoter. Lac repressor appears to control transcription by preventing RNA polymerase and/or CAP protein from binding to the lac promoter. Results support the idea that the lac promoter is composed of two sites that interact with CAP protein and RNA polymerase holoenzyme. The promoter can be altered by mutation so that holoenzyme alone can initiate lac transcription correctly.

Keywords: cyclic AMP, RNA polymerase, sigma factor, rho factor, cAMP-binding protein

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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