Abstract
Infection of mouse embryo cells with two strains of murine sarcoma virus and one strain of murine leukemia virus was followed rapidly by synthesis of DNA in the cytoplasm. Persistently infected cells have not shown such synthesis, and ultraviolet-irradiated virus did not induce DNA synthesis. This new DNA presumably represents an intermediate in the virus replication cycle specified by the virion DNA polymerase(s). Failure to observe cytoplasmic DNA synthesis in persistently infected cells suggests, in keeping with the results of inhibitor experiments, that the new “viral” DNA becomes associated with cellular DNA in some form of stable interaction.
Keywords: mouse embryo fibroblasts, murine sarcoma virus, Rauscher murine leukemia virus, autoradiography
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- BADER J. P. THE ROLE OF DEOXYRIBONUCLEIC ACID IN THE SYNTHESIS OF ROUS SARCOMA VIRUS. Virology. 1964 Apr;22:462–468. doi: 10.1016/0042-6822(64)90067-4. [DOI] [PubMed] [Google Scholar]
- Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
- Boettiger D., Temin H. M. Light inactivation of focus formation by chicken embryo fibroblasts infected with avian sarcoma virus in the presence of 5-bromodeoxyuridine. Nature. 1970 Nov 14;228(5272):622–624. doi: 10.1038/228622a0. [DOI] [PubMed] [Google Scholar]
- Comings D. E., Kakefuda T. Initiation of deoxyribonucleic acid replication at the nuclear membrane in human cells. J Mol Biol. 1968 Apr 14;33(1):225–229. doi: 10.1016/0022-2836(68)90290-8. [DOI] [PubMed] [Google Scholar]
- Hirschman S. Z., Fischinger P. J., O'Connor T. E. Effect of infection with Moloney sarcoma and leukemia viruses on nucleic acid synthesis in mouse cell cultures. J Natl Cancer Inst. 1970 Jan;44(1):107–116. [PubMed] [Google Scholar]
- Nass M. M. Mitochondrial DNA: advances, problems, and goals. Science. 1968 Jul 4;165(3888):25–35. [PubMed] [Google Scholar]
- TEMIN H. M. THE PARTICIPATION OF DNA IN ROUS SARCOMA VIRUS PRODUCTION. Virology. 1964 Aug;23:486–494. doi: 10.1016/0042-6822(64)90232-6. [DOI] [PubMed] [Google Scholar]
- Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]