Abstract
In the presence of over-threshold concentrations of simple neutral polymers and salts, DNA undergoes a cooperative change in its solution structure. Sedimentation studies at low DNA concentrations show that phage DNA molecules collapse into particles approaching the compactness of the contents of phage heads. The interaction between DNA and polymers is thought to be nonspecifically replusive.
Keywords: poly(vinyl pyrrolidinone), poly(ethylene oxide), T4 phage, centrifugation
Full text
PDF![1886](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c27/389314/01de0ac123d3/pnas00083-0227.png)
![1887](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c27/389314/1825cb792e1d/pnas00083-0228.png)
![1888](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c27/389314/e8d93edc5f13/pnas00083-0229.png)
![1889](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c27/389314/3dc2976e391c/pnas00083-0230.png)
![1890](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c27/389314/f4f337b80ca1/pnas00083-0231.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dubin S. B., Benedek G. B., Bancroft F. C., Freifelder D. Molecular weights of coliphages and colip- hage DNA. II. Measurement of diffusion coefficients using optical mixing spectroscopy, and measurement of sedimentation coefficients. J Mol Biol. 1970 Dec 28;54(3):547–556. doi: 10.1016/0022-2836(70)90125-7. [DOI] [PubMed] [Google Scholar]
- Eiserling F. A., Geiduschek E. P., Epstein R. H., Metter E. J. Capsid size and deoxyribonucleic acid length: the petite variant of bacteriophage T4. J Virol. 1970 Dec;6(6):865–876. doi: 10.1128/jvi.6.6.865-876.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Giannoni G., Padden F. J., Jr, Keith H. D. Crystallization of DNA from dilute solution. Proc Natl Acad Sci U S A. 1969 Mar;62(3):964–971. doi: 10.1073/pnas.62.3.964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gray H. B., Jr, Hearst J. E. Flexibility of native DNA from the sedimentation behavior as a function of molecular weight and temperature. J Mol Biol. 1968 Jul 14;35(1):111–129. doi: 10.1016/s0022-2836(68)80041-5. [DOI] [PubMed] [Google Scholar]
- Gray H. B., Jr Sedimentation coefficient of polyoma virus DNA. Biopolymers. 1967;5(10):1009–1019. doi: 10.1002/bip.1967.360051012. [DOI] [PubMed] [Google Scholar]
- Lang D. Collapse of single DNA molecules in ethanol. J Mol Biol. 1969 Nov 28;46(1):209–209. doi: 10.1016/0022-2836(69)90069-2. [DOI] [PubMed] [Google Scholar]
- STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]