Table 2. In vitro Fab/ligand sample concentrations for assay optimization experiments.

High concentration samples			Low concentration samples
Fab concentration (nM)	Ligand concentration (nM)		Fab concentration (nM)	Ligand concentration (nM)
500	10		0.3	0.012
350	10		0.2	0.012
200	10		0.1	0.012
120	10		0.05	0.012
80	10		0.025	0.012
40	10		0.015	0.012
20	10		0.010	0.012
9.2	10		0.005	0.012