Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Sep;68(9):2066–2068. doi: 10.1073/pnas.68.9.2066

Insulin-Sepharose and the Dynamics of Insulin Action

Takami Oka 1, Yale J Topper 1
PMCID: PMC389352  PMID: 5289366

Abstract

In terms of the rate of accumulation of α-aminoisobutyric acid, mammary epithelial cells from mature virgin mice respond rapidly to insulin covalently bound to sepharose particles, but do not respond initially to soluble insulin in vitro. The response to insulin-sepharose is prevented if the cells are first exposed to insulin. These observations may provide new insight into the dynamics of insulin action.

Keywords: mammary epithelial cells, mice, α-aminoisobutyric-acid accumulation

Full text

PDF
2066

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cuatrecasas P. Interaction of insulin with the cell membrane: the primary action of insulin. Proc Natl Acad Sci U S A. 1969 Jun;63(2):450–457. doi: 10.1073/pnas.63.2.450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Friedberg S. H., Oka T., Topper Y. J. Development of insulin-sensitivity by mouse mammary gland in vitro. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1493–1500. doi: 10.1073/pnas.67.3.1493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Porath J., Axen R., Ernback S. Chemical coupling of proteins to agarose. Nature. 1967 Sep 30;215(5109):1491–1492. doi: 10.1038/2151491a0. [DOI] [PubMed] [Google Scholar]
  4. Rodbell M., Birnbaumer L., Pohl S. L., Krans H. M. The glucagon-sensitive adenyl cyclase system in plasma membranes of rat liver. V. An obligatory role of guanylnucleotides in glucagon action. J Biol Chem. 1971 Mar 25;246(6):1877–1882. [PubMed] [Google Scholar]
  5. Topper Y. J. Multiple hormone interactions related to the growth and differentiation of mammary gland in vitro. Trans N Y Acad Sci. 1968 Apr;30(6):869–874. doi: 10.1111/j.2164-0947.1968.tb02530.x. [DOI] [PubMed] [Google Scholar]
  6. Voytovich A. E., Owens I. S., Topper Y. J. A novel action of insulin on phosphoprotein formation by mammary gland explants. Proc Natl Acad Sci U S A. 1969 May;63(1):213–217. doi: 10.1073/pnas.63.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES