Abstract
Individual pairs of human chromosomes can be reliably identified by a new method that does not require special optical equipment and that results in permanent preparations. This method, which is based on treatment of the chromosomes in situ with NaOH, followed by incubation in sodium chloride-trisodium citrate and Giemsa staining, results in highly specific banding patterns in characteristic regions of the chromosome arms. It should prove useful for the detection of small structural changes in chromosomes.
Keywords: heterochromatin, Giemsa stain, chromosome bands
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arrighi F. E., Hsu T. C. Localization of heterochromatin in human chromosomes. Cytogenetics. 1971;10(2):81–86. doi: 10.1159/000130130. [DOI] [PubMed] [Google Scholar]
- Arrighi F. E., Hsu T. C., Saunders P., Saunders G. F. Localization of repetitive DNA in the chromosomes of Microtus agrestis by means of in situ hybridization. Chromosoma. 1970;32(2):224–236. doi: 10.1007/BF00286011. [DOI] [PubMed] [Google Scholar]
- Caspersson T., Lomakka G., Moller A. Computerized chromosome identification by aid of the quinacrine mustard fluorescence technique. Hereditas. 1972;67(1):103–110. doi: 10.1111/j.1601-5223.1971.tb02364.x. [DOI] [PubMed] [Google Scholar]
- Caspersson T., Lomakka G., Zech L. The 24 fluorescence patterns of the human metaphase chromosomes - distinguishing characters and variability. Hereditas. 1972;67(1):89–102. doi: 10.1111/j.1601-5223.1971.tb02363.x. [DOI] [PubMed] [Google Scholar]
- Caspersson T., Zech L., Johansson C. Differential binding of alkylating fluorochromes in human chromosomes. Exp Cell Res. 1970 Jun;60(3):315–319. doi: 10.1016/0014-4827(70)90523-9. [DOI] [PubMed] [Google Scholar]
- Pardue M. L., Gall J. G. Chromosomal localization of mouse satellite DNA. Science. 1970 Jun 12;168(3937):1356–1358. doi: 10.1126/science.168.3937.1356. [DOI] [PubMed] [Google Scholar]
- Waring M., Britten R. J. Nucleotide sequence repetition: a rapidly reassociating fraction of mouse DNA. Science. 1966 Nov 11;154(3750):791–794. doi: 10.1126/science.154.3750.791. [DOI] [PubMed] [Google Scholar]