Figure 1.

Culture of adherent monocytes with interleukin-4 (IL-4) and granulocyte–macrophage colony-stimulating factor (GM-CSF) results in a characteristic dendritic cell (DC) phenotype. Monocytes were isolated from human peripheral blood by sequential density gradient centrifugations followed by adhesion in serum-free medium. Adherent monocytes were then washed twice with PBS and cultured in medium containing IL-4 and GM-CSF for 7 days. After 7 days, cells were harvested and immunolabelled with monoclonal antibodies against the indicated molecule or isotype control and analysed by flow cytometry. A representative scatter plot and histogram overlays are shown; grey = isotype control.