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. 1971 Sep;68(9):2231–2235. doi: 10.1073/pnas.68.9.2231

Transcription of the Polyoma Virus Genome: Synthesis and Cleavage of Giant Late Polyoma-Specific RNA

Nicholas H Acheson *,†, Elena Buetti *,†, Klaus Scherrer *, Roger Weil *,†
PMCID: PMC389391  PMID: 4332250

Abstract

The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized “late” polyoma-specific RNA appears as “giant” molecules of heterogeneous size, up to several times larger than a strand of polyoma DNA (1.5 × 106 daltons). Treatment with dimethylsulfoxide or urea showed that the large size of these molecules is not due to aggregation. Giant polyoma-specific RNA is strikingly similar in size distribution to “nuclear messenger-like” RNA (“heterogeneous nuclear” RNA) of the host cell. Subsequent to its synthesis, some of the giant polyoma-specific RNA appears to be cleaved to at least three smaller species.

Keywords: gel electrophoresis, density gradient centrifugation, RNA-DNA hybridization, tumor virus

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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