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. 1971 Sep;68(9):2252–2257. doi: 10.1073/pnas.68.9.2252

A Novel Closed-Circular Mitochondrial DNA with Properties of a Replicating Intermediate

Harumi Kasamatsu 1,2, Donald L Robberson 1,2, Jerome Vinograd 1,2
PMCID: PMC389395  PMID: 5289384

Abstract

A fraction of the covalently closed mitochondrial DNA in mouse L cells contains a replicated heavy-strand segment that is hydrogen bonded to the circular light strand. The inserted single strand is dissociable from the circular duplex at an elevated temperature.

Keywords: density-gradient centrifugation, electron microscopy, separated strands, ethidium bromide, mouse DNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer W., Vinograd J. Interaction of closed circular DNA with intercalative dyes. II. The free energy of superhelix formation in SV40 DNA. J Mol Biol. 1970 Feb 14;47(3):419–435. doi: 10.1016/0022-2836(70)90312-8. [DOI] [PubMed] [Google Scholar]
  2. Bauer W., Vinogradj The interaction of closed circular DNA with intercalative dyes. 3. Dependence of the buoyant density upon superhelix density and base composition. J Mol Biol. 1970 Dec 14;54(2):281–298. doi: 10.1016/0022-2836(70)90430-4. [DOI] [PubMed] [Google Scholar]
  3. Clayton D. A., Davis R. W., Vinograd J. Homology and structural relationships between the dimeric and monomeric circular forms of mitochondrial DNA from human leukemic leukocytes. J Mol Biol. 1970 Jan 28;47(2):137–153. doi: 10.1016/0022-2836(70)90335-9. [DOI] [PubMed] [Google Scholar]
  4. Clayton D. A., Smith C. A., Jordan J. M., Teplitz M., Vinograd J. Occurrence of complex mitochondrial DNA in normal tissues. Nature. 1968 Dec 7;220(5171):976–979. doi: 10.1038/220976a0. [DOI] [PubMed] [Google Scholar]
  5. Eason R., Vinograd J. Superhelix density heterogeneity of intracellular simian virus 40 deoxyribonucleic acid. J Virol. 1971 Jan;7(1):1–7. doi: 10.1128/jvi.7.1.1-7.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LITTLEFIELD J. W. THREE DEGREES OF GUANYLIC ACID--INOSINIC ACID PYROPHOSPHORYLASE DEFICIENCY IN MOUSE FIBROBLASTS. Nature. 1964 Sep 12;203:1142–1144. doi: 10.1038/2031142a0. [DOI] [PubMed] [Google Scholar]
  7. Lee C. S., Davis R. W., Davidson N. A physical study by electron microscopy of the terminally reptitious, circularly permuted DNA from the coliphage particles of Escherichia coli 15. J Mol Biol. 1970 Feb 28;48(1):1–22. doi: 10.1016/0022-2836(70)90215-9. [DOI] [PubMed] [Google Scholar]
  8. Robberson D., Aloni Y., Attardi G. Electron microscopic visualization of mitochondrial RNA-DNA hybrids. J Mol Biol. 1971 Jan 28;55(2):267–270. doi: 10.1016/0022-2836(71)90196-3. [DOI] [PubMed] [Google Scholar]
  9. Wetmur J. G., Davidson N. Kinetics of renaturation of DNA. J Mol Biol. 1968 Feb 14;31(3):349–370. doi: 10.1016/0022-2836(68)90414-2. [DOI] [PubMed] [Google Scholar]

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