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. 1971 Sep;68(9):2308–2311. doi: 10.1073/pnas.68.9.2308

Changes in Macromolecular Synthesis Associated with the Induction of Glutamine Synthetase in Embryonic Retina

P K Sarkar 1, A A Moscona 1
PMCID: PMC389406  PMID: 4399928

Abstract

The hydrocortisone-mediated induction of glutamine synthetase in the neural retina of chicken embryo in vitro is correlated with enhanced incorporation into protein of [14C]aspartic acid, an amino acid abundant in this enzyme. In the induced retina labeled with [14C]aspartic acid, a peak of radioactivity was detected in the region of the polysomal profile corresponding to polysomes comprising 12-14 ribosomes. In retinas labeled with [3H]uridine, an increased amount of radioactivity was also detected in the same polysomal region of the hydrocortisone-induced retina. If we assume a monocistronic messenger RNA for retinal glutamine synthetase, this region corresponds to the estimated size of the polysomes necessary for the translation of this enzyme. The evidence presented demonstrates a correlation between these changes in incorporation and the induction of glutamine synthetase.

Keywords: hydrocortisone, differentiation, polysomes, cytosine arabinoside

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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