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. 1971 Oct;68(10):2321–2324. doi: 10.1073/pnas.68.10.2321

N-Terminal Amino-Acid Sequence of African Lungfish Immunoglobulin Light Chains

G W Litman *, A C Wang †, H H Fudenberg †, R A Good *
PMCID: PMC389412  PMID: 5289864

Abstract

The sequence of the N-terminal ten amino acids of the unblocked light chains derived from the low molecular weight immunoglobulin of a dipnoid fish, the African lungfish (Protopterus aethiopicus), has been determined. A degree of sequence heterogeneity as extensive as that displayed by pooled mammalian light chains was encountered. The major N-terminal sequence of the amino acids of light chains from lungfish immunoglobins can be made homologous with that of various Elasmobranchean, Chondrostean, Avian, and Mammalian species if an internal deletion of two base triplets in the gene coding for light chains from lungfish immunoglobulins is assumed. The existence of a deletion and/or insertion mechanism may have profound biological significance, since it would be ideal for the introduction of variability into immunoglobulin chains without sacrificing certain integral features of the antibody molecule.

Keywords: Protopterus aethiopicus, 5.8 and 19S, κ and λ chains

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appella E. Amino acid sequences of two mouse immunoglobulin lambda chains. Proc Natl Acad Sci U S A. 1971 Mar;68(3):590–594. doi: 10.1073/pnas.68.3.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Edelman G. M., Gall W. E. The antibody problem. Annu Rev Biochem. 1969;38:415–466. doi: 10.1146/annurev.bi.38.070169.002215. [DOI] [PubMed] [Google Scholar]
  3. Edman P., Begg G. A protein sequenator. Eur J Biochem. 1967 Mar;1(1):80–91. doi: 10.1007/978-3-662-25813-2_14. [DOI] [PubMed] [Google Scholar]
  4. Frangione B., Milstein C. Partial deletion in the heavy chain disease protein ZUC. Nature. 1969 Nov 8;224(5219):597–599. doi: 10.1038/224597a0. [DOI] [PubMed] [Google Scholar]
  5. Franklin E. C., Frangione B. The molecular defect in a protein (CRA) found in gamma-1 heavy chain disease, and its genetic implications. Proc Natl Acad Sci U S A. 1971 Jan;68(1):187–191. doi: 10.1073/pnas.68.1.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goodman J. W., Klaus G. G., Nitecki D. E., Wang A. C. Pyrrolidonecarboxylic acid at the N-terminal positions of polypeptide chains from leopard shark immunoglobulins. J Immunol. 1970 Jan;104(1):260–262. [PubMed] [Google Scholar]
  7. Gros C., Labouesse B. Study of the dansylation reaction of amino acids, peptides and proteins. Eur J Biochem. 1969 Feb;7(4):463–470. doi: 10.1111/j.1432-1033.1969.tb19632.x. [DOI] [PubMed] [Google Scholar]
  8. Hill R. L., Delaney R., Fellows R. E., Lebovitz H. E. The evolutionary origins of the immunoglobulins. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1762–1769. doi: 10.1073/pnas.56.6.1762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hood L. E., Potter M., McKean D. J. Immunoglobulin structure: amino terminal sequences of kappa chains from genetically similar mice (BALB/c). Science. 1970 Dec 11;170(3963):1207–1210. doi: 10.1126/science.170.3963.1207. [DOI] [PubMed] [Google Scholar]
  10. Hood L., Eichmann K., Lackland H., Krause R. M., Ohms J. J. Rabbit antibody light chains and gene evolution. Nature. 1970 Dec 12;228(5276):1040–1044. doi: 10.1038/2281040a0. [DOI] [PubMed] [Google Scholar]
  11. Kubo R. T., Rosenblum I. Y., Benedict A. A. The unblocked N-terminal sequence of chicken IgG lambda-like light chains. J Immunol. 1970 Aug;105(2):534–536. [PubMed] [Google Scholar]
  12. Litman G. W., Fromme D., Chartrand S. L., Finstad J., Good R. A. Significance of heavy chain mass and antigenic relationship in immunoglobulin evolution. Immunochemistry. 1971 Apr;8(4):345–349. doi: 10.1016/0019-2791(71)90156-x. [DOI] [PubMed] [Google Scholar]
  13. Marchalonis J. J. Isolation and characterization of immunoglobulin-like proteins of the Australian lungfish (Neoceratodus forsteri). Aust J Exp Biol Med Sci. 1969 Aug;47(4):405–419. doi: 10.1038/icb.1969.46. [DOI] [PubMed] [Google Scholar]
  14. Neville D. M., Jr Fractionation of cell membrane protein by disc electrophoresis. Biochim Biophys Acta. 1967 Jan 18;133(1):168–170. doi: 10.1016/0005-2795(67)90051-7. [DOI] [PubMed] [Google Scholar]
  15. Novotný J., Franek F., Sorm F. Large scale isolation, characterization and classification of pig immunoglobulin kappa-chains. Eur J Biochem. 1970 Jun;14(2):309–316. doi: 10.1111/j.1432-1033.1970.tb00291.x. [DOI] [PubMed] [Google Scholar]
  16. Pink R., Wang A. C., Fudenberg H. H. Antibody variability. Annu Rev Med. 1971;22:145–170. doi: 10.1146/annurev.me.22.020171.001045. [DOI] [PubMed] [Google Scholar]
  17. Pisano J. J., Bronzert T. J. Analysis of amino acid phenylthiohydantoins by gas chromatography. J Biol Chem. 1969 Oct 25;244(20):5597–5607. [PubMed] [Google Scholar]
  18. Pollara B., Suran A., Finstad J., Good R. A. N-terminal amino acid sequences of immunoglobulin chains in Polyodon spathula. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1307–1312. doi: 10.1073/pnas.59.4.1307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Smithies O., Gibson D. M., Fanning E. M., Percy M. E., Parr D. M., Connell G. E. Deletions in immunoglobulin polypeptide chains as evidence for breakage and repair in DNA. Science. 1971 May 7;172(3983):574–577. doi: 10.1126/science.172.3983.574. [DOI] [PubMed] [Google Scholar]
  20. Suran A. A., Papermaster B. W. N-terminal sequences of heavy and light chains of leopard shark immunoglobulins: evolutionary implications. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1619–1623. doi: 10.1073/pnas.58.4.1619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Terry W. D., Ohms J. Implications of heavy chain disease protein sequences for multiple gene theories of immunoglobulin synthesis. Proc Natl Acad Sci U S A. 1970 Jun;66(2):558–563. doi: 10.1073/pnas.66.2.558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wang A. C., Pink J. R., Fudenberg H. H., Ohms J. A variable region subclass of heavy chains common to immunoglobulins G, A, and M and characterized by an unblocked amino-terminal residue. Proc Natl Acad Sci U S A. 1970 Jul;66(3):657–663. doi: 10.1073/pnas.66.3.657. [DOI] [PMC free article] [PubMed] [Google Scholar]

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