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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Oct;68(10):2341–2344. doi: 10.1073/pnas.68.10.2341

The Elastomeric Rack in Biology

Henry Eyring *, Frank H Johnson
PMCID: PMC389417  PMID: 5289868

Abstract

A model designated “The elastomeric rack” is discussed with reference to the mechanism of enzyme catalysis and the necessity of large protein molecules in the process. This model, which represents an extension of the earlier “Rack mechanism,” stems from experimental evidence demonstrating large volume changes of activation or of reaction accompanying various biochemical reactions and more complicated physiological processes. For reasons discussed, the volume changes, as revealed through the influence of increased hydrostatic pressure on reaction rates and equilibria, prove that there are large conformational changes in the protein which, because of numerous cross links in a complex network, lead to additive stress on the substrate-enzyme complex, and thereby to increased reactivity. The manner in which the model serves to account for the influence of changes in the environment (temperature, pressure, chemical composition, electrical fields) on the activity of the protein catalyst, and the modifying action of particular inhibitory or activating substances, alone or in combination, is briefly discussed. The bearing of various considerations on protein denaturation, excitation of nerve, contraction of muscle, specific precipitation, and the divers physiological processes in living cells, including mutation, cancer, and the degenerative diseases of aging, is also briefly discussed.

Keywords: conformational changes, pressure, activation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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