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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Oct;68(10):2374–2376. doi: 10.1073/pnas.68.10.2374

Quantitative Analysis of Urine Vapor and Breath by Gas-Liquid Partition Chromatography

Linus Pauling *, Arthur B Robinson *, Roy Teranishi , Paul Cary *
PMCID: PMC389425  PMID: 5289873

Abstract

When a human being is placed for several days on a completely defined diet, consisting almost entirely of small molecules that are absorbed from the stomach into the blood, intestinal flora disappear because of lack of nutrition. By this technique, the composition of body fluids can be made constant (standard deviation about 10%) after a few days, permitting significant quantitative analyses to be performed. A method of temperature-programmed gas-liquid partition chromatography has been developed for this purpose. It permits the quantitative determination of about 250 substances in a sample of breath, and of about 280 substances in a sample of urine vapor. The technique should be useful in the application of the principles of orthomolecular medicine.

Keywords: orthomolecular medicine, vitamins, controlled diet

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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