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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Oct;68(10):2386–2390. doi: 10.1073/pnas.68.10.2386

Activation and Isolation of Hamster-Specific C-Type RNA Viruses from Tumors Induced by Cell Cultures Transformed by Chemical Carcinogens

Aaron E Freeman *, Gary J Kelloff , Raymond V Gilden , William T Lane , Ansel P Swain §, Robert J Huebner
PMCID: PMC389428  PMID: 5289874

Abstract

Cell cultures of Syrian hamster embryo were treated for 7 days with selected chemicals. Certain cultures were morphologically transformed by three different chemical preparations and yielded cell lines that subsequently produced malignant tumors in hamsters. Although the cell lines were negative for infectious virus before inoculation into animals, hamster-specific C-type RNA virus was isolated from tumors or from cell lines derived from the tumors. Since infectious C-type viruses are usually not demonstrable in hamster tissues of normal or tumor origin, we conclude that the chemical treatment and activation of the viruses are related events.

Keywords: hamster-embryo fibroblasts, 3-methylcholanthrene, cigarette-smoke condensate

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Selected References

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