Abstract
Cell-free protein synthesizing extracts incubated with purified preparations of colicin E3 showed a marked decrease of their protein synthesizing activity. Incubation of ribosomes prepared from S-30 extracts with E3 resulted in their inactivation. Their 16S RNA was found to lose a terminal fragment, similar in size to the fragment released upon in vivo treatment with E3. Extracts derived from colicinogenic strains are resistant to the action of E3in vitro.
Keywords: E. coli, immune strains, S30, 16S RNA
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