Abstract
C55-isoprenoid alcohol phosphokinase, a butanol-soluble enzyme from the membrane of Staphylococcus aureus, has been purified to homogeneity by the inclusion of organic solvents in all of the techniques used. By polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, the enzyme is a single polypeptide chain of molecular weight 17,000. The amino-acid analysis reveals an unusually high content of nonpolar amino acids (58%), the largest amount so far reported in any protein. When equilibrated in butanol-water, enzyme apoprotein is found in the butanol layer. The influence of sodium chloride indicates that the lipid requirement of the enzyme is nonelectrostatic in nature.
Keywords: Staphylococcus aureus, nonpolar amino acid
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- Andrews P. Estimation of molecular size and molecular weights of biological compounds by gel filtration. Methods Biochem Anal. 1970;18:1–53. [PubMed] [Google Scholar]
- DAS M. L., CRANE F. L. PROTEOLIPIDS. I. FORMATION OF PHOSPHOLIPID-CYTOCHROME C COMPLEXES. Biochemistry. 1964 May;3:696–700. doi: 10.1021/bi00893a017. [DOI] [PubMed] [Google Scholar]
- Hatch F. T. Correlation of amino-acid composition with certain characteristics of proteins. Nature. 1965 May 22;206(4986):777–779. doi: 10.1038/206777a0. [DOI] [PubMed] [Google Scholar]
- Heller J. Structure of visual pigments. I. Purification, molecular weight, and composition of bovine visual pigment500. Biochemistry. 1968 Aug;7(8):2906–2913. doi: 10.1021/bi00848a030. [DOI] [PubMed] [Google Scholar]
- Higashi Y., Siewert G., Strominger J. L. Biosynthesis of the peptidoglycan of bacterial cell walls. XIX. Isoprenoid alcohol phosphokinase. J Biol Chem. 1970 Jul 25;245(14):3683–3690. [PubMed] [Google Scholar]
- Higashi Y., Strominger J. L. Biosynthesis of the peptidoglycan of bacterial cell walls. XX. Identification of phosphatidylglycerol and cardiolipin as cofactors for isoprenoid alcohol phosphokinase. J Biol Chem. 1970 Jul 25;245(14):3691–3696. [PubMed] [Google Scholar]
- Rothfield L., Romeo D. Role of lipids in the biosynthesis of the bacterial cell envelope. Bacteriol Rev. 1971 Mar;35(1):14–38. doi: 10.1128/br.35.1.14-38.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]