Fig. 3.

MHC expression by NPCs in response to JHMV infection. Cultured GFP NPCs were infected with JHMV (m.o.i.=0.1) for 18 h and subsequently replenished with media alone or containing 100 U/mL IFN-γ. Cells were incubated for 24 h and MHC class I/II expression of non-infected and infected NPCs evaluated via flow cytometry. The frequency of NPCs expressing either MHC class I (A) or MHC class II (B) is shown in representative dot plots. (C) Quantification of the frequency of MHC I/II expression by infected or non-infected NPCs treated with media alone or in combination with IFN-γ. Data is presented as average±SEM and represents three independent experiments (^⁎p<0.05). (D) Representative dot plots of MHC II-positive NPCs versus JHMV-positive NPCs.