Abstract
RNA preparations from dark-grown, light-grown, and greening Euglena gracilis have been compared by polyacrylamide gel electrophoresis and by hybridization to Euglena chloroplast DNA. Chloroplast ribosomal RNA is not detected in dark-grown cells; its abundance increases in greening cells over a 72 hr period until the concentration characteristic of light-grown cells is reached. Other RNA species complementary to chloroplast DNA are present in comparable abundance in light-grown, dark-grown, and greening cells.
Keywords: polyacrylamide gel electrophoresis, DNA-RNA hybridization-competition
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- Bendich A. J., McCarthy B. J. Ribosomal RNA homologies among distantly related organisms. Proc Natl Acad Sci U S A. 1970 Feb;65(2):349–356. doi: 10.1073/pnas.65.2.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bolle A., Epstein R. H., Salser W., Geiduschek E. P. Transcription during bacteriophage T4 development: synthesis and relative stability of early and late RNA. J Mol Biol. 1968 Feb 14;31(3):325–348. doi: 10.1016/0022-2836(68)90413-0. [DOI] [PubMed] [Google Scholar]
- Brown R. D., Haselkorn R. Synthesis and maturation of cytoplasmic ribosomal RNA in Euglena gracilis. J Mol Biol. 1971 Aug 14;59(3):491–503. doi: 10.1016/0022-2836(71)90312-3. [DOI] [PubMed] [Google Scholar]
- Heizmann P. Propriétés des ribosomes et des RNA ribosomiques d'Euglena gracilis. Biochim Biophys Acta. 1970 Nov 12;224(1):144–154. [PubMed] [Google Scholar]
- Pickard B. G., Thimann K. V. Transport and Distribution of Auxin during Tropistic Response. II. The Lateral Migration of Auxin in Phototropism of Coleoptiles. Plant Physiol. 1964 May;39(3):341–350. doi: 10.1104/pp.39.3.341. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Portier C., Nigon V. Propriétés des RNA ribosomiques obtenus à partir d'une forme balnche d'Euglena gracilis. Biochim Biophys Acta. 1968 Dec 17;169(2):540–542. [PubMed] [Google Scholar]
- Schiff J. A., Zeldin M. H., Rubman J. Chlorophyll Formation and Photosynthetic Competence in Euglena During Light-Induced Chloroplast Development in the Presence of 3, (3,4-dichlorophenyl) 1,1-Dimethyl Urea (DCMU). Plant Physiol. 1967 Dec;42(12):1716–1725. doi: 10.1104/pp.42.12.1716. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stutz E., Rawson J. R. Separation and characterization of Euglena gracilis chloroplast single-strand DNA. Biochim Biophys Acta. 1970 May 21;209(1):16–23. doi: 10.1016/0005-2787(70)90656-8. [DOI] [PubMed] [Google Scholar]